BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
狂犬病毒的 G 蛋白是一种三聚体，大约 67kD，是诱导生成病毒中和抗体(virus-neutralizing antibodies，VNA)的主要抗原，并且可以诱导机体产生对狂犬病毒致命感染的免疫。G 蛋白同时含有毒力决定簇。G 基因是第一种被克隆和测序的狂犬病毒基因。 本试剂盒采用间接法酶联免疫吸附试验（ELISA）。往预先包被有犬抗狂犬病毒IgG(RV-IgG)抗原的微孔中，依次加入样本、阳性对照、阴性对照、HRP标记的二抗，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的犬抗狂犬病毒IgG(RV-IgG)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），判断样本阴阳性。
This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Dog RV-IgG antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Dog RV-IgG in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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