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非洲猪瘟（英文名称：Infection with African swine fever virus，简称： ASF）是由非洲猪瘟病毒（英文名称：African Swine fever virus，简称： ASFV）感染家猪和各种野猪（如非洲野猪、欧洲野猪等）而引起的一种急性、 出血性、烈性传染病。世界动物卫生组织（OIE）将其列为法定报告动物疫病。其特征是发病过程短，最急性 和急性感染死亡率高达 100%，临床表现为发热（达 40～42℃），心跳加快， 呼吸困难，眼、鼻有浆液性或粘液性脓性分泌物，皮肤发绀，淋巴结、肾、胃肠粘膜出血，非洲猪瘟临床症状与猪瘟症状相似，只能依靠实验室监测确诊。 本试剂盒采用间接法酶联免疫吸附试验（ELISA）。往预先包被有非洲猪瘟抗体(ASF-Ab)抗原的微孔中，依次加入样本、阳性对照、阴性对照、HRP标记的二抗，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的非洲猪瘟抗体(ASF-Ab)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），判断样本阴阳性。
This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Pig ASF-Ab antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Pig ASF-Ab in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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