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狂犬病毒 N 蛋白是组成病毒粒子的主要核蛋白,是诱导狂犬病细胞免疫的主要成分,常用于狂犬病病毒的诊断、分类和流行病学研究。其基因长 1424 个核苷酸,序列相对稳定,点突变较少,是狂犬病病毒基因分型的主要依据。N 蛋白的磷酸化调节病毒的转录和复制;它与基因组 RNA 结合,保护病毒 RNA 免受核酸酶的破坏;在成熟的病毒颗粒中,它也是核壳螺旋对称结构的主要组成部分。 本试剂盒采用间接法酶联免疫吸附试验(ELISA)。往预先包被有小鼠抗狂犬病毒N蛋白抗体抗原的微孔中,依次加入样本、阳性对照、阴性对照、HRP标记的二抗,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠抗狂犬病毒N蛋白抗体呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),判断样本阴阳性。
This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse Anti-Rabies virus N protein antibody antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Mouse Anti-Rabies virus N protein antibody in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.