BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
乙肝核心抗体，也叫抗-HBc，英文缩写是 HBcAb。它也是乙型肝炎急性感 染的早期标志，在血清中存在的时间很长。在 HBsAg 和抗-HBs 阴性时，能 从血清中检出乙肝核心抗体，高滴度的乙肝核心抗体也是一种传染标志。抗 —HBc 可分为三型：抗—HBcIgM、抗—HBcIgA、抗—HBcIgG，在 HBV 感染中这几个型依次出现，也会依次消失，只有 IgG 这一型可长期存在。 本试剂盒采用间接法酶联免疫吸附试验（ELISA）。往预先包被有人乙型肝炎病毒核心抗体IgM(HBcAb-IgM)抗原的微孔中，依次加入样本、阳性对照、阴性对照、HRP标记的二抗，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人乙型肝炎病毒核心抗体IgM(HBcAb-IgM)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），判断样本阴阳性。
This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human HBcAb-IgM antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Human HBcAb-IgM in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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