计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
刚地弓形虫是一种能引发弓形虫病的寄生性囊泡虫,分布于世界各地,几乎能感染所有温血动物,但猫科动物是其唯一已知的终宿主,在猫体内可进行有性繁殖。在啮齿动物体内,刚地弓形虫会改变其行为,增加其被猫科动物捕食的机会。在人类中,尤其是婴儿和免疫力低下者,刚地弓形虫感染通常无症状,但可能会导致严重的弓形虫病。刚地弓形虫在感染后的最初几周可能会引起轻微的类似流感的症状,但除此之外,健康的成年人通常没有症状。 本试剂盒采用间接法酶联免疫吸附试验(ELISA)。往预先包被有人弓形虫IgM(Tox-IgM)抗原的微孔中,依次加入样本、阳性对照、阴性对照、HRP标记的二抗,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人弓形虫IgM(Tox-IgM)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),判断样本阴阳性。
This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human Tox-IgM antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Human Tox-IgM in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.