BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
梅毒螺旋体是梅毒的病原体，因其透明，不易着色，故又称苍白螺旋体。梅毒螺旋体只感染人类，分获得性梅毒与胎传梅毒。获得性梅毒主要通过性接触传染；胎传梅毒由梅毒螺旋体通过胎盘，从脐带血循环传给胎儿，可引起胎儿全身感染。螺旋体在胎儿内脏及组织中大量繁殖，可引起胎儿死亡或流产。 本试剂盒采用间接法酶联免疫吸附试验（ELISA）。往预先包被有人梅毒螺旋体抗体(TP-Ab)抗原的微孔中，依次加入样本、阳性对照、阴性对照、HRP标记的二抗，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人梅毒螺旋体抗体(TP-Ab)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），判断样本阴阳性。
This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human TP-Ab antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Human TP-Ab in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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