脑组织的标本较难取得，尤其是某些疾病模型或特殊药物处理的难度更高。在对脑组织进行观察分析时，由于实验结果的不可预估性以及实验方案的易变性，决定了研究人员随时需要脑片进行补充试验，因此，无需使用低温冰箱但能完好保存脑片的低成本切片冻存液就显得尤为重要。
冰冻切片冻存液又称组织切片冻存液、切片冻存液、切片防冻液或切片抗冻缓冲液(anti-freeze buffer, AFB)，用于冰冻切片的长期冷冻保存。可较长时间保存组织切片的抗原成份和酶活性，是形态学研究领域较好的保存冰冻切片的方法。本产品由蔗糖、聚乙烯比咯烷酮、乙二醇、磷酸盐等组成，需低温冷冻保存。因含有蔗糖和乙二醇等抗冻剂，-20℃条件下，组织切片不会被冻结或形成冰晶，可完好保存至少6个月。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤(仅供参考)：
1、在24孔板或48孔板中加入1-2ml的冰冻切片冻存液。
2、组织经OCT冷冻包埋剂包埋、切片，用预冷的细毛笔将切下的切片收集于孔板中，置于-20℃条件下保存。
3、实验前，将孔板从-20℃条件下取出，于室温条件下放置10min左右，加入4℃预冷的PBS室温下漂洗10min，再加入室温存放的PBS漂洗两次，每次5min，漂洗完即可进行相应的染色操作。
注意事项：
1、本品含有蔗糖，不能长期0℃以上保存，如发生霉变或产生絮状物则不能使用。
2、试剂开封后请尽快使用。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Brain tissue samples are difficult to obtain, especially those from certain disease models or treated with specific drugs, which pose even greater challenges. When observing and analyzing brain tissue, the unpredictability of experimental results and the variability of experimental protocols mean researchers often need additional brain sections for supplementary tests. Therefore, a low-cost section cryopreservation solution that can preserve brain sections intact without requiring a low-temperature refrigerator becomes particularly important.
Frozen Section Cryopreservation Solution, also known as Tissue Section Cryopreservation Solution, Section Cryopreservation Solution, Section Antifreeze Solution, or Section Antifreeze Buffer (AFB), is used for the long-term frozen storage of frozen sections. It can preserve the antigen components and enzyme activity of tissue sections for an extended period, making it an excellent method for preserving frozen sections in the field of morphological research. This product consists of sucrose, polyvinylpyrrolidone, ethylene glycol, phosphate, and other components, and requires low-temperature frozen storage. Due to the presence of antifreeze agents such as sucrose and ethylene glycol, tissue sections will not freeze or form ice crystals at -20°C and can be preserved intact for at least 6 months. This reagent is for research use only and not intended for clinical diagnosis or other purposes.
Operating Procedures (for reference only)
1. Add 1-2 ml of Frozen Section Cryopreservation Solution to a 24-well plate or 48-well plate.
2. After embedding the tissue with OCT freezing embedding medium and sectioning, collect the cut sections into the well plate using a pre-cooled fine brush, and store the plate at -20°C.
3. Before the experiment, remove the well plate from -20°C, let it stand at room temperature for approximately 10 minutes. Add PBS pre-cooled to 4°C and rinse at room temperature for 10 minutes, then add PBS stored at room temperature and rinse twice, 5 minutes each time. After rinsing, the corresponding staining operation can be performed.
Precautions
1. This product contains sucrose and cannot be stored above 0°C for a long time. Do not use it if mold grows or flocculent substances appear.
2. Please use the reagent as soon as possible after opening.
3. For your safety and health, wear a lab coat and disposable gloves during operation.
ZJ26F0838282
危险品化学品经营许可证（带存储）