福尔马林色素是产生于酸性福尔马林固定组织过程中的棕色或棕黑色的沉积物,多出现于血液丰富的组织、出血的损伤区域以及充满血的大血管,多表现为一种微晶沉积物,具有非单折射性。
传统的从切片去除福尔马林色素的方法有苦味酸、氢氧化钠、氢氧化钾等方法,但以上方法有可能影响后续染色过程。Aladdin福尔马林色素清除液相较以上方法对组织的损伤更小,色素沉着也较少;长时间固定富含血液的器官,福尔马林色素沉着会大大增加,这时建议更换固定液。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考)
1、切片入70%乙醇。
2、入福尔马林色素清除液处理 5~15min(见注意事项1)。
3、蒸馏水冲洗干净,进行下游染色。
注意事项
1、根据福尔马林色素的量,调整去除时间。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用,以防影响后续实验效果。
Formalin pigment is a brown or brown-black deposit produced during the fixation of tissues in acidic formalin. It commonly appears in blood-rich tissues, hemorrhagic lesion areas, and large blood vessels filled with blood. It typically presents as microcrystalline deposits and is non-birefringent.
Principle:
Traditional methods for removing formalin pigment from sections include picric acid, sodium hydroxide, and potassium hydroxide. However, these methods may affect subsequent staining processes. Compared to these methods, the Aladdin Formalin Pigment Removal Solution causes less damage to tissue and results in less pigment deposition. Prolonged fixation of blood-rich organs significantly increases formalin pigment deposition; in such cases, it is recommended to replace the fixative. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Procedure (For Reference Only)
1. Place the section into 70% ethanol.
2. Treat with the Formalin Pigment Removal Solution for 5 to 15 minutes (see Note 1).
3. Rinse thoroughly with distilled water and proceed with downstream staining.
Precautions
1. Adjust the treatment time based on the amount of formalin pigment present.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
3. After opening the reagent, please use it as soon as possible to prevent affecting subsequent experimental results.