计算溶液所需的质量、体积或浓度。
BioReagent,分子生物学级,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细菌鞭毛是细菌的运动器官,幽门螺杆菌能够从强酸性的胃内腔穿过胃上皮细胞上的黏液层达到胃上皮细胞的中性环境,这就是鞭毛运动作用的很好例证,通过鞭毛染色可以观察到鞭毛形态、数量和鞭毛在菌体分布的位置,鞭毛数量和在菌体上的分布位置是鉴定细菌的重要依据之一。
阿拉丁鞭毛染色液 (Loffler法) 采用Loffler法,又称罗夫蓝染色,试剂操作简单,结果判断更可靠。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
产品组分及储存条件:
| F1510199 | Component | 2×50ml | Storage |
| F1510199A | Loffler染色液 | 50 mL | RT |
| F1510199B | 结晶紫染色液 | 50 mL | RT. Store in the dark. |
自备材料:
酒精灯、载玻片、接种环、显微镜、蒸馏水
操作步骤(仅供参考):
1、细菌悬液涂片,加热固定或35°C恒温箱内干燥固定。
2、将玻片入Loffler染色液,取出加热干燥1min。
3、95%乙醇冲洗。
4、将玻片入结晶紫染色液,取出加热干燥1min。
5、水洗,空气中干燥。
6、镜检:从涂片边缘开始,由外及里,逐渐移至中心;细菌分布少的地方,鞭毛容易观察;细菌密集的地方,鞭毛被菌体挡住,不易观察。
染色结果:
菌体和鞭毛呈不同程度的红色。
注意事项:
1、玻片应洁净,无油污。
2、染色的菌种应连续传代多次,处于生长活跃期。
3、染色过程应小心操作,防止鞭毛脱落。
4、固定时不宜用高热火焰固定。
5、为了您的安全和健康,请穿实验服并戴一次性手套操作。
6、试剂开封后请尽快使用,以防影响后续实验效果。
Bacterial flagella are the motile organs of bacteria. Helicobacter pylori can penetrate the mucus layer on gastric epithelial cells from the strongly acidic gastric lumen to reach the neutral microenvironment around the gastric epithelial cells, which is a good example of the motile function of flagella. Flagella staining enables the observation of flagellar morphology, quantity, and their distribution on bacterial cells. The number and distribution of flagella on bacterial cells are among the important criteria for bacterial identification.
Aladdin Flagella Stain Solution (Löffler Method), which adopts the Löffler Method and is also known as Löffler’s Flagella Stain, features simple operation and more reliable result interpretation. This reagent is intended for research purposes only and is not suitable for clinical diagnosis or any other applications.
Product Components and Storage Conditions:
| F1510199 | Component | 2×50ml | Storage |
| F1510199A | Loffler Stain Solution | 50 mL | RT |
| F1510199B | Crystal Violet Stain Solution | 50 mL | RT. Store in the dark. |
Materials to Be Prepared:
Alcohol lamp, glass slide, inoculation loop, microscope, distilled water
Operating Procedures (For Reference Only):
1. Prepare a smear of the bacterial suspension, then fix it by heating or by air-drying in a 35°C incubator.
2. Immerse the glass slide in Löffler Stain Solution, then take it out and heat-dry for 1 minute.
3. Rinse the slide with 95% ethanol.
4. Immerse the glass slide in Crystal Violet Stain Solution, then take it out and heat-dry for 1 minute.
5. Rinse the slide with distilled water and air-dry at room temperature.
6. Microscopic examination: Start from the edge of the smear and move gradually from the periphery to the center. Flagella are easier to observe in areas with sparse bacterial distribution; in densely populated bacterial areas, flagella are obscured by the bacterial cells and thus difficult to observe.
Staining Results:
Bacterial cells and flagella exhibit varying degrees of red color.
Precautions:
1. Glass slides should be clean and free of oil stains.
2. The strains to be stained should be subcultured continuously for multiple generations and maintained in the logarithmic growth phase.
3. Handle with care during the staining process to prevent flagella detachment.
4. Avoid high-temperature flame fixation during the fixing step.
5. For your safety and health, wear a lab coat and disposable gloves when performing the experiment.
6. Use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | F1510199 |
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