细菌鞭毛是细菌的运动器官，幽门螺杆菌能够从强酸性的胃内腔穿过胃上皮细胞上的黏液层达到胃上皮细胞的中性环境，这就是鞭毛运动作用的很好例证，通过鞭毛染色可以观察到鞭毛形态、数量和鞭毛在菌体分布的位置，鞭毛数量和在菌体上的分布位置是鉴定细菌的重要依据之一。
阿拉丁鞭毛染色液采用石碳酸复红法，该染色法的优点是采用石碳酸复红作为核心染料，试剂比较稳定，操作简单，结果判断更可靠。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
F1510205
| Component
| 100 mL
| Storage
|
F1510205A
| 染色稀释液
| 100 mL
| RT. Store in the dark.
|
F1510205B
| 碱性品红染色液
| 10 mL
| RT
|
自备材料：
1、蒸馏水
2、载玻片、显微镜、接种环、恒温箱
操作步骤(仅供参考)：
1、配制鞭毛染色工作液：使用前按试剂(A):试剂(B)=10:1混合，即为鞭毛染色工作液，室温保存待用。
2、在洁净无油脂的载玻片上滴加2滴蒸馏水。
3、用接种环挑取无菌蒸馏水，再与血平板上菌落接触，允许细菌游到接种环蒸馏水中，再将接种环移到玻片上蒸馏水顶部轻点2次。
4、轻轻摇动玻片，使细菌分布均匀；切勿研磨和搅动，以防鞭毛脱落。
5、置室温或35°C恒温箱内干燥固定。
6、滴加鞭毛染色工作液染色10～15min。
7、轻轻水洗，自然晾干。
8、镜检：从涂片边缘开始，由外及里，逐渐移至中心；细菌分布少的地方，鞭毛容易观察；细菌密集的地方，鞭毛被菌体挡住，不易观察。
染色结果：
菌体和鞭毛皆为红色，菌体染色较鞭毛为深。
注意事项：
1、固定时不宜用火焰固定。
2、为了您的安全和健康，请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用，以防影响后续实验效果。
Bacterial flagella are the motile organs of bacteria. Helicobacter pylori can penetrate the mucus layer on the gastric epithelial cells from the strongly acidic gastric lumen to reach the neutral microenvironment around the gastric epithelial cells, which is a good example of the motile function of flagella. Flagella staining enables the observation of flagellar morphology, quantity, and their distribution on bacterial cells. The number and distribution of flagella on bacterial cells are among the important criteria for bacterial identification.
Aladdin Flagella Stain Solution adopts the Carbol Fuchsin Method. The advantage of this staining method is that it uses carbol fuchsin as the core dye, which ensures relatively stable reagents, simple operation, and more reliable result interpretation. This reagent is intended for research purposes only and is not suitable for clinical diagnosis or any other applications.
Product Components and Storage Conditions:
F1510205
| Component
| 100 mL
| Storage
|
F1510205A
| Staining Diluent
| 100 mL
| RT. Store in the dark.
|
F1510205B
| Basic Fuchsin Stain Solution
| 10 mL
| RT
|
Materials to Be Prepared:
1. Distilled water
2. Glass slides, microscope, inoculation loop, incubator
Operating Procedures (For Reference Only):
1. Prepare the flagella staining working solution: Mix Reagent (A) and Reagent (B) at a ratio of 10:1 before use to obtain the flagella staining working solution. Store it at room temperature for later use.
2. Place 2 drops of distilled water onto a clean, grease-free glass slide.
3. Use an inoculation loop to take a small amount of sterile distilled water, then touch the colonies on the blood agar plate to allow the bacteria to swim into the distilled water on the loop. Next, gently touch the top of the distilled water drops on the glass slide twice with the inoculation loop.
4. Shake the glass slide slightly to distribute the bacteria evenly; do not grind or stir vigorously to prevent flagella detachment.
5. Air-dry and fix the smear at room temperature or in a 35°C incubator.
6. Add the flagella staining working solution dropwise and stain for 10–15 minutes.
7. Rinse the slide gently with water and air-dry naturally.
8. Microscopic examination: Start from the edge of the smear and move gradually from the periphery to the center. Flagella are easier to observe in areas with sparse bacterial distribution; in densely populated bacterial areas, flagella are obscured by the bacterial cells and thus difficult to observe.
Staining Results:
Both bacterial cells and flagella stain red, with the color of bacterial cells being deeper than that of flagella.
Precautions:
1. Avoid flame fixation during the fixing step.
2. For your safety and health, wear a lab coat and disposable gloves during operation.
3. Use the reagent as soon as possible after opening to prevent it from affecting the results of subsequent experiments.
ZJ26F0333152
危险品化学品经营许可证（带存储）