计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
1,6-二磷酸果糖 (FDP) 是糖酵解过程中的中间产物,具有调节糖代谢中某些酶活性的功能,为恢复、改善细胞代谢的分子水平药物,广泛应用于医药、保健、美容等行业。 本试剂盒提供一种简单,灵敏,快速的测定方法:1,6-二磷酸果糖 (FDP)在醛缩酶(ALD)和磷酸甘油醛异构酶(TIM)的作用下生成磷酸两分子二羟丙酮(DAP),DAP 在 3-磷酸甘油脱氢酶 (GDH) 作用下能将NADH 氧化,通过检测 NADH 在 340nm 处的下降量,进而计算得出 1,6-二磷酸果糖 (FDP)含量。
Fructose 1,6-bisphosphate (FBP/FDP) is an intermediate product in the glycolytic pathway. It functions to regulate the activity of certain enzymes in sugar metabolism and serves as a molecular-level drug for restoring and improving cellular metabolism. Consequently, it is widely used in the pharmaceutical, healthcare, and cosmetic industries. This kit provides a simple, sensitive, and rapid assay method: Fructose 1,6-bisphosphate (FDP) is converted into two molecules of dihydroxyacetone phosphate (DHAP) under the action of aldolase (ALD) and triosephosphate isomerase (TIM). Subsequently, DHAP oxidizes NADH in the presence of glycerol-3-phosphate dehydrogenase (GDH). The content of fructose 1,6-bisphosphate (FDP) can be calculated by measuring the decrease in NADH at 340 nm.