计算溶液所需的质量、体积或浓度。
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甲酰胺上样缓冲液 (10×) 为10 倍浓缩 RNA 专用上样缓冲液,主要由去离子甲酰胺、溴酚蓝、EDTA 等组成,以溴酚蓝作为电泳指示剂,稀释至 1× 后比重较大,样品可快速沉降至加样孔,电泳条带迁移清晰易观察。使用时将样品与缓冲液混合即可,无需进一步稀释,仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考)
将 RNA 样品与甲酰胺上样缓冲液 (10×) 按1:9的比例混合。
直接加入 RNA 变性凝胶加样孔中进行电泳。
注意事项
若单次使用量较小,建议分装后使用,降低污染风险。
实验全程严格避免 RNase 污染。
操作时请穿戴实验服及一次性手套,保证实验安全。
试剂开封后请尽快密封使用,以免影响实验效果。
Formamide Loading Buffer (10×) is a 10-fold concentrated loading buffer specifically designed for RNA electrophoresis. It is primarily composed of deionized formamide, bromophenol blue, EDTA, and other components, with bromophenol blue serving as the electrophoresis tracking dye. When diluted to 1×, the buffer has a relatively high density, allowing samples to quickly settle into the wells, resulting in clear and easily observable electrophoretic band migration. For use, simply mix the sample with the buffer; no further dilution is required. For research use only. Not for use in diagnostic procedures or other purposes.
Procedure (For Reference Only)
Mix the RNA sample with Formamide Loading Buffer (10×) at a ratio of 1:9.
Directly load the mixture into the wells of a denaturing RNA gel for electrophoresis.
Notes
If the single-use volume is small, it is recommended to aliquot the reagent before use to reduce the risk of contamination.
Strictly avoid RNase contamination throughout the entire experimental procedure.
Please wear laboratory appropriate attire and disposable gloves during operation to ensure experimental safety.
Use the reagent as soon as possible after opening and keep it sealed to avoid affecting experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | F1518788 |
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