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Hephaestin (HP)作为铜蓝蛋白的同系物,是近年来发现的铁转运蛋白亚铁氧化酶，HP属亚铁氧化酶家族成员,具有亚铁氧化酶的活性,参与体内铁代谢。HP的表达可受铁、铜及锌等金属离子的调节。HP催化Fe²⁺氧化生成Fe³⁺，在介导铁的跨膜转运中有重要作用。 测定原理：HP催化Fe²⁺氧化为Fe³⁺，Fe²⁺和ferrozine反应显色，在560 nm下有特征吸光值。通过测定Fe²⁺的减少速率可测得亚铁氧化酶的活性。
Hephaestin (HP), a homolog of ceruloplasmin, is a recently discovered ferroxidase involved in iron transport. HP belongs to the ferroxidase family and possesses ferroxidase activity, participating in iron metabolism in the body. The expression of HP can be regulated by metal ions such as iron, copper, and zinc. HP catalyzes the oxidation of Fe²⁺ to Fe³⁺ and plays an important role in mediating transmembrane iron transport. Determination Principle: HP catalyzes the oxidation of Fe²⁺ to Fe³⁺. Fe²⁺ reacts with ferrozine to produce a colored product with a characteristic absorption peak at 560 nm. Ferroxidase activity can be determined by measuring the rate of decrease in Fe²⁺ concentration.
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