甲醛是一种能与蛋白质、核酸和脂类产生非特异性反应的活泼化合物，对所有生物都具有很高毒性。甲醛脱氢酶作为含锌中等链醇脱氢酶（ADH）的家庭成员之一，广泛存在于原核和真核生物中，该酶能利用NAD⁺作为辅酶，将有毒的甲醛氧化，是甲醛氧化途径中的关键酶。 测定原理：甲醛脱氢酶催化甲醛和NAD⁺产生NADH，在340nm处的吸光值会增加，测定340nm处的吸光值变化，可计算得到甲醛脱氢酶的活性。
Formaldehyde is a reactive compound that non-specifically reacts with proteins, nucleic acids, and lipids, and is highly toxic to all organisms. Formaldehyde dehydrogenase, a member of the zinc-containing medium-chain alcohol dehydrogenase (ADH) family, is widely present in prokaryotes and eukaryotes. This enzyme utilizes NAD⁺ as a cofactor to oxidize toxic formaldehyde and is a key enzyme in the formaldehyde oxidation pathway. Determination Principle: Formaldehyde dehydrogenase catalyzes the reaction between formaldehyde and NAD⁺ to produce NADH, resulting in an increase in absorbance at 340 nm. The activity of formaldehyde dehydrogenase can be calculated by measuring the change in absorbance at 340 nm.
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