Formate dehydrogenase (FDH, EC 1.2.1.2) belongs to the class of D-2-hydroxy acid dehydrogenases and is widely used in the regeneration of coenzyme NADH.
This kit utilizes formate dehydrogenase (FDH) to catalyze the irreversible reaction of formate and NAD⁺ to generate carbon dioxide and NADH. By measuring the rate of NADH generation at 340 nm, the activity of formate dehydrogenase (FDH) can be calculated.