计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
果糖-1,6-二磷酸酶(Fructose 1,6-bisphosphatase,FBP)又称果糖-1,6-二磷酸酯酶,其在糖异生过程和光合作用同化物蔗糖的合成中起关键性的作用。催化 1,6-二磷酸果糖和水生成 6-磷酸果糖和无机磷。该产品可检测动植物组织、细胞等生物样本。在该试剂盒中,FBP 催化 1,6-二磷酸果糖和水,生成6-磷酸果糖和无机磷,在反应体系中添加的磷酸葡萄糖异构酶和 6-磷酸葡萄糖脱氢酶依次催化生成 6-磷酸葡萄糖酸和 NADPH,测定340 nm下NADPH的增加速率,即可计算 FBP 活性。
Fructose-1,6-bisphosphatase (FBP), also known as fructose-1,6-diphosphatase, plays a key role in gluconeogenesis and the synthesis of sucrose, a photosynthate, in photosynthetic organisms. It catalyzes the conversion of fructose-1,6-bisphosphate and water into fructose-6-phosphate and inorganic phosphorus. This product can be used to detect FBP activity in biological samples such as animal and plant tissues, cells, and more. In this assay kit, FBP catalyzes the reaction of fructose-1,6-bisphosphate with water to produce fructose-6-phosphate and inorganic phosphorus. Subsequently, phosphoglucose isomerase and glucose-6-phosphate dehydrogenase, added to the reaction system, sequentially catalyze the conversion to generate 6-phosphogluconate and NADPH. By measuring the rate of increase in NADPH at 340 nm, FBP activity can be calculated.