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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
果糖-1,6二磷酸酶又称果糖1,6二磷酸酯酶,催化1,6二磷酸果糖和水生成6磷酸果糖和无机磷,在糖的异生代谢和光合作用同化物蔗糖的合成中起关键性的作用。 测定原理:FBP催化1,6二磷酸果糖和水生成6磷酸果糖和无机磷,在反应体系中添加的磷酸葡萄糖异构酶和6-磷酸葡萄糖脱氢酶依次催化生成6-磷酸葡萄糖酸和NADPH,340nm下测定NADPH增加速率,即可计算FBP活性。
Fructose-1,6-bisphosphatase (FBP) catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate, playing a key role in gluconeogenesis and the synthesis of sucrose as a photosynthate. Determination Principle: FBP catalyzes the conversion of fructose-1,6-bisphosphate and water to fructose-6-phosphate and inorganic phosphate. Phosphoglucose isomerase and glucose-6-phosphate dehydrogenase added to the reaction system sequentially catalyze the formation of 6-phosphogluconate and NADPH. FBP activity is calculated by measuring the rate of NADPH increase at 340 nm.