BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血红蛋白(Hemoglobin，Hb或HGB)是高等生物体内负责运载氧的一种蛋白质，是能使血液呈红色的蛋白，血红蛋白由四条链组成，两条α链和两条β链，每一条链有一个包含一个铁原子的环状血红素，Hb在氧含量高的区域容易与氧结合，在氧含量低的区域又容易与氧分离，血红蛋白的这一特性，使红细胞具有运输氧的功能，血管溶血时血浆游离血红蛋白(free hemoglobin)浓度增高。 该试剂盒检测原理是血红蛋白中亚铁血红素有类似过氧化物酶的作用，可在氧化剂的作用下催化邻联甲苯胺(O-tolidine)产生青色或蓝色产物，吸收峰在630nm；在酸性条件下产物呈黄色，吸收峰在435nm，产物黄色越深，说明FHB含量越高，反之越低；通过分光光度比色法测定435nm处吸光度，就可计算出血浆游离血红蛋白含量，主要用于血浆样本，亦可用于测定细胞或组织的裂解液或匀浆液等样品中游离血红蛋白含量。本产品仅用于科研领域，不适用于临床诊断或其他用途。
Hemoglobin (Hb or HGB) is a protein responsible for oxygen transport in higher organisms and is the protein that gives blood its red color. Hemoglobin is composed of four polypeptide chains—two alpha chains and two beta chains—with each chain containing a ring-like heme group that includes an iron atom. Hb readily binds to oxygen in areas with high oxygen concentration and readily dissociates from oxygen in areas with low oxygen concentration. This property of hemoglobin enables red blood cells to transport oxygen. When intravascular hemolysis occurs, the concentration of plasma free hemoglobin (FHB) increases. The detection principle of this kit is as follows: The ferrous heme in hemoglobin exhibits peroxidase-like activity and can catalyze the oxidation of O-tolidine in the presence of an oxidizing agent, producing a cyan or blue product with an absorption peak at 630 nm. Under acidic conditions, the product appears yellow with an absorption peak at 435 nm. The deeper the yellow color of the product, the higher the FHB content, and vice versa. By measuring the absorbance at 435 nm using spectrophotometric colorimetry, the plasma free hemoglobin content can be calculated. This kit is primarily used for plasma samples and can also be used to determine free hemoglobin content in samples such as cell or tissue lysates or homogenates. This product is for research use only and is not intended for use in diagnostic procedures.
危险品化学品经营许可证（带存储）