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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
纤维素酶是由微生物产生的多组分的酶系，能水解纤维素β-1,4葡萄糖苷键生成葡萄糖，研究滤纸酶活力对纤维素酶的研究具有非常重要的意义。 测定原理：滤纸酶水解滤纸产生的还原糖能与3,5-二硝基水杨酸生成红棕色氨基化合物，在540nm处有最大光吸收，在一定范围内反应液颜色深浅与还原糖的量成正比，可测定计算得滤纸酶的活力。
Cellulase is a multi-component enzyme system produced by microorganisms. It hydrolyzes the β-1,4-glucosidic bonds in cellulose to produce glucose. Studying filter paper activity is of great significance for cellulase research. Determination Principle: The reducing sugars produced by the hydrolysis of filter paper catalyzed by filter paper activity react with 3,5-dinitrosalicylic acid to form a reddish-brown amino compound, which exhibits maximum absorbance at 540 nm. Within a certain range, the color intensity of the reaction solution is proportional to the amount of reducing sugars, allowing the filter paper activity to be determined and calculated.
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