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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
延胡索酸酶又名延胡索酸水化酶(EC 4.2.1.2),存在于线粒体中的富马酸酶是柠檬酸循环中的关键酶之一,存在于胞质中的富马酸酶与氨基酸和富马酸酯的代谢关系密切。在人类中,该酶缺失会导致严重的健康问题,例如胎儿脑畸形,肌张力低下等。 延胡索酸酶催化延胡索酸转化成L-苹果酸,L-苹果酸在苹果酸脱氢酶的作用下,同时使NAD⁺还原成NADH,通过检测NADH在340nm的增加速率得出延胡索酸酶的活性大小。
Fumarase, also known as fumarate hydratase (EC 4.2.1.2), is a key enzyme in the citric acid cycle when located in the mitochondria, while the cytosolic form is closely associated with the metabolism of amino acids and fumarate esters. In humans, deficiency of this enzyme can lead to serious health issues, such as fetal brain malformations and hypotonia. Fumarase catalyzes the conversion of fumarate to L-malate. Under the action of malate dehydrogenase, L-malate simultaneously reduces NAD⁺ to NADH. The activity of fumarase is determined by measuring the rate of increase of NADH at 340 nm.