α-1,2-Fucosyltransferase (WbgL), EC 2.4.1.69, is derived from Escherichia coli O126. This enzyme attaches fucose to the glycan chain of Galβ1,4GlcOR via an α-1,2 glycosidic linkage and acts as the key enzyme for the catalytic synthesis of 2'-fucosyllactose. WbgL preferentially recognizes galactoside-linked substrates. The product is heterologously expressed in Escherichia coli.
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Precautions
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