计算溶液所需的质量、体积或浓度。
EnzymoPure™,His标签,生物活性,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
α-1,6-岩藻糖苷酶突变体 (E274A) 是α 1,6-岩藻糖苷酶的突变体,E.C 3.2.1.51,基因来源于Lactobacillus casei,可用于GlcNAc-peptides 上连接岩藻糖,与LpAlfC(WT)功能互逆。本产品为大肠杆菌重组表达。
反应式
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使用方法
1. 按照反应体系加入底物,可适当加入缓冲盐调节pH为7.4,加入适量LpAlfC(E274A)(根据反应需求);
2. 混匀后,将样品置于37℃孵育2-4h或者过夜。
【注】体系放大时,孵育时间或酶量需要根据实际情况调整,可低速震荡混匀。
【注】多数常见的生物缓冲液适用,比如 Tris 或 PBS,不在此 pH 范围的缓冲液(如醋酸缓冲液)可能适用,但是孵育时间或酶量需要根据实际情况进行优化。
为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途!
α-1,6-fucosidase(E274A) is a mutant of α‑1,6‑fucosidase (E.C. 3.2.1.51), with the gene derived from Lactobacillus casei. It can be used to attach fucose to GlcNAc‑peptides, and its function is the reverse of that of LpAlfC (wild‑type). This product is recombinantly expressed in E. coli.
Reaction equation
Usage Instructions
1.Add substrates according to the reaction system. Buffer salts may be added to adjust the pH to 7.4 if necessary, then add an appropriate amount of LpAlfC(E274A) (depending on the reaction requirements).
2.Mix well and incubate the sample at 37 °C for 2–4 hours or overnight.
Notes
1.When scaling up the reaction system, the incubation time or enzyme amount should be adjusted according to actual conditions; low‑speed shaking may be used to ensure mixing.
2.Most common biological buffers, such as Tris or PBS, are suitable. Buffers outside this pH range (e.g., acetate buffers) may also be applicable, but the incubation time or enzyme amount will need to be optimized based on the specific conditions.
Safety and Usage Restriction
For your health, please wear a lab coat and disposable gloves during handling. This product is for research use only!
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