BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
果糖-1,6-二磷酸酶（FBP，EC 3.1.3.11）是糖异生途径的关键酶。多种糖异生前体经系列酶促反应转化为果糖-1,6-二磷酸，后者在FBP催化下水解为果糖-6-磷酸和无机磷。该酶的异常表达与多种疾病密切相关。本试剂盒提供一种简单、灵敏、快速的测定方法：FBP催化果糖-1,6-二磷酸水解生成果糖-6-磷酸和无机磷，与偶联酶体系作用，生成的NADPH与特异性显色探针反应产生有色物质，通过监测该有色物质的生成速率，即可计算FBP的活性。
Fructose-1,6-bisphosphatase (FBP, EC 3.1.3.11) is a key enzyme in the gluconeogenesis pathway. Various gluconeogenic precursors are converted to fructose-1,6-bisphosphate through a series of enzymatic reactions, which is then hydrolyzed by FBP to fructose-6-phosphate and inorganic phosphate (Pi). Aberrant expression of this enzyme is closely associated with a variety of diseases. This kit provides a simple, sensitive, and rapid assay for the determination of FBP activity: FBP catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate, which, through a coupled enzyme system, generates NADPH. The NADPH reacts with a specific chromogenic probe to produce a colored compound. By monitoring the rate of formation of this colored compound, FBP activity can be calculated.
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