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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
类黄酮糖基转移酶能够催化UDPG与槲皮素反应，生成UDP和槲皮素糖苷。UDP在丙酮酸激酶与乳酸脱氢酶的依次作用下，氧化NADH生成NAD⁺。NAD⁺的生成速度与UDP含量成正比，而NADH在340 nm处具有特征吸收峰，通过测定该波长下吸光度的变化速率，即可定量表征类黄酮糖基转移酶的活性。
Flavonoid glycosyltransferase catalyzes the reaction of UDP-glucose (UDPG) with quercetin to generate UDP and quercetin glycoside. Through the sequential actions of pyruvate kinase and lactate dehydrogenase, the resulting UDP drives the oxidation of NADH to NAD⁺. The rate of NAD⁺ generation is proportional to the UDP content, and since NADH exhibits a characteristic absorption peak at 340 nm, the enzyme activity can be quantitatively determined by monitoring the rate of change in absorbance at this wavelength.
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