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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
亚铁离子(Fe²⁺)与亚铁嗪(Ferrozine)结合,形成稳定的紫红色络合物,该络合物在562 nm波长处具有最大吸收峰。当样品中存在螯合剂时,螯合剂会优先与Fe²⁺结合,导致可供显色剂反应的游离Fe²⁺减少,从而降低显色反应体系的吸光度。根据样品在562 nm处吸光度的大小,即可评价其对亚铁离子的螯合能力。
Ferrous ions (Fe²⁺) react with Ferrozine to form a stable purple‑red complex, which exhibits maximum absorption at a wavelength of 562 nm. When a chelating agent is present in the sample, it preferentially binds to Fe²⁺, thereby reducing the amount of free Fe²⁺ available to react with the chromogenic reagent, and consequently decreasing the absorbance of the colorimetric reaction system. Based on the absorbance of the sample at 562 nm, the chelating ability toward ferrous ions can be evaluated.