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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
酸溶短链脂酰基肉碱经处理后水解为游离肉碱,肉碱与乙酰辅酶A(Acetyl-CoA)在肉碱乙酰转移酶(CrAT)催化下生成游离态辅酶A(CoASH),CoASH与DTNB反应生成TNB,TNB在412 nm处具有特征吸收峰,通过吸光值变化即可定量检测游离肉碱与总肉碱的含量。
Acid‑soluble short‑chain acylcarnitines are hydrolyzed to free carnitine upon treatment. In the presence of carnitine acetyltransferase (CrAT), carnitine reacts with acetyl‑CoA to form free coenzyme A (CoASH). CoASH then reacts with DTNB to generate TNB, which exhibits a characteristic absorption peak at 412 nm. The content of free carnitine and total carnitine can be quantitatively determined by measuring the change in absorbance.