BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
游离脂肪酸（FFA），亦称非酯化脂肪酸，在血液中主要与白蛋白结合后循环运输，是反映动物机体脂类代谢状况的重要生理生化指标。血清FFA浓度与脂类代谢、糖代谢及内分泌功能密切相关，在糖尿病、严重肝功能障碍、甲状腺功能亢进等疾病状态下常出现异常升高。本试剂盒采用有机溶剂萃取法提取FFA，萃取液中的FFA与三乙醇胺铜反应，在有机相中生成脂肪酸铜（铜皂）复合物；复合物中的Cu²⁺进一步与显色液反应生成紫红色络合物，其颜色深浅与Cu²⁺浓度成正比（符合朗伯-比尔定律），通过测定该产物在特征波长处的吸光度即可定量计算样品中FFA的含量。
Free fatty acids (FFA), also known as non‑esterified fatty acids, are primarily transported in the blood bound to albumin and serve as important physiological and biochemical indicators reflecting the lipid metabolism status of animals. Serum FFA concentrations are closely related to lipid metabolism, glucose metabolism, and endocrine function, and are often abnormally elevated in pathological conditions such as diabetes mellitus, severe liver dysfunction, and hyperthyroidism. This kit employs an organic solvent extraction method to isolate FFA. The FFA in the extract reacts with copper triethanolamine to form a copper fatty acid (copper soap) complex in the organic phase. The Cu²⁺ ions in the complex subsequently react with a chromogenic reagent to generate a purple‑red complex. The color intensity is proportional to the Cu²⁺ concentration in accordance with the Lambert‑Beer law. By measuring the absorbance of this product at a characteristic wavelength, the FFA content in the sample can be quantitatively determined.
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