计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,充氩,干燥。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
FITC-DEAE-dextran is designed for special studies on membrane and transport properties in cells and tissues. Cationic (positively charged) diethylaminoethyl groups have been introduced into standard FITC-dextran. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. Aladdin produce FITC-DEAE-dextrans from 4 kDa to 150 kDa. FITC-DEAE-dextrans are supplied as a yellow powder.Polysucrose are renamed from Ficoll™.
Structure
FITC-DEAE-dextrans are synthesized from well-characterised dextran fractions derived from Leuconostoc mesenteroides B512F. The degree of substitution for FITC lies between 0.001 – 0.008 and the limits for nitrogen content are 3-5% – corresponding to about one DEAE-substituent per three glucose units. The synthesis of DEAE-dextran introduces two types of DEAE-substituents into the dextran chain (see structure in datafile). The predominant substituent is the single tertiary group, but this reacts further particularly at higher degree of substitution to yield a “tandem” group containing a quaternary amine as well as the tertiary amine.
Spectral data
The excitation maximum for FITC-DEAE-dextran is 495 nm and the emission maximum is 520 nm. Maximum intensity is observed at pH 9.
Application
FITC-CM-dextran is mainly used for studying permeability and microcirculation. The diethylaminoethyl groups will impart an overall positive charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues.
| Excitation and Emision Ranges | 495 nm and 520 nm |
|---|