计算溶液所需的质量、体积或浓度。
Factor I
有货
为什么选择此级别
PBS Only,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-80℃储存,避免反复冻融。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Purity
>95% by SDS-PAGE
Deficiencies
Extinction Coeff.
A280 nm = 1.43 at 1.0 mg/mL
A280 nm = 1.43 at 1.0 mg/mL
Precautions
Use normal precautions for handling human blood products
General Description
Human factor I is purified from normal human serum (Fearon, D.T. (1977)).
Factor I is a naturally glycosylated serine protease which cleaves and inactivates C3b and C4b. Factor I is highly specific but inactive without a cofactor such as the soluble factor H and C4b binding protein (C4BP) (Pangburn, M.K. et al (1977)). Membrane-bound cofactors include CR1 (complement receptor 1 (CD35) which is found on human red cells and other l ymphoid cells) and MCP (membrane cofactor protein (CD46) which is found on most human nucleated cells). Factor I cleaves the alpha-peptide chain of C3b and C4b when these are bound to one of the cofactors. This cleavage inactivates all of the complement activating functions of these proteins producing iC3b and iC4b. Factor I can cleave the alpha chain of C3b twice and this releases a small fragment called C3f (17 amino acids, ~3 kDa). In the presence of CR1 factor can cleave iC3b releasing C3c from C3dg. C4b is cleaved rapidly at two sites separating C4c from C4d. Nomenclature for this protein has changed over time and it has been called C3b inactivator (C3b-INA), C3b/C4b inactivator, and conglutinin-activating factor (KAF).
Physical Characteristics & Structure
Molecular weight: 88,000 daltons composed of 2 disulfide-linked chains of 50,000 and 38,000 daltons. It is synthesized as a single chain molecule. The protein is heavily glycosylated with at least 6 glycosylation sites (3 on each chain) ( Morley, B.J. et al. (2000)). The total carbohydrate content is approximately 10.7 %. The protein is
negatively charged at serum pH and exhibits a heterogeneous pI = 4.5 to 6.0 due primarily to differences in sialic acid content.
MDL Number: MFCD00166552
EC Number: EC 3.4.21.45
Function
See general description above. Even though it is a serine protease with trypsinlike specificity for Lys/Arg, it exhibits virtually no activity against peptide or ester substrates and its activity is only very slowly inactivated by DFP (diisopropyl fluorophosphate) or other serine protease class inhibitors. It is thought to have an inactive conformation except when bound in complex with its substrates and cofactors.
Assays
The most convenient assay measures the cleavage of substrates C3b or C4b by SDS gels. This must be done in the presence of the appropriate cofactor: factor H (for C3b) and C4BP (for C4b) (Morgan, B.P. (2000)). A typical C3b cleavage assay should contain approximately 4 µg C3b, 1 µg factor H and various amounts of factor I from 0 to 1 µg in a total volume of 15 µL. A typical C4b cleavage assay should contain approximately 4 µg C4b, 1 µg C4BP and various amounts of factor I from 0 to 0.1 µg in a total volume of 15 µL. The assays should be set up on wet ice, then incubated for 10 min at 37℃ at which time SDS sample buffer containing a reducing agent should be added to the tubes and the samples heated for 5 min. SDS PAGE gels run under reducing conditions should reveal cleavage of the alpha chains of the respective protein. A continuously monitored fluorescent assay has been reported (Pangburn, M.K. et al. (1983)) which takes advantage of the approximately 10-fold drop in fluorescence of ANS (8-anilino-1-naphthalenesulfonic acid) in the presence of C3b when that C3b is converted to iC3b.
Applications
Deficiencies have been treated for acute crises with fresh frozen plasma transfusions as well as fractions of plasma enriched in factor I.
In vivo
The average concentration is 34 µg/mL (0.39 µM) in human plasma. The protein is produced primarily in the liver although mRNA as well as protein expression has been identified in monocytes, endothelial cells, fibroblasts, muscle and nerve cells.
Regulation
Factor I plasma concentration increases with infections and it is one of the acute phase proteins. Synthesis in tissue cells is increased by bacterial lipopolysaccharide (LPS) and by gamma interferon.
Genetics
The gene for factor I is located on human chromosome 4q25. The gene spans 63 kb with 13 exons (CFI(3426)). In the mouse it is on chromosome 3 at 66.6 cM. Accession numbers: Human (cDNA: Y00318, J02770; Genomic X78594), Mouse (U47810).
Deficiencies are autosomal recessive and are characterized by low levels of functional C3 due to uncontrolled complement activation (Ziegler , J.B. et al. (1975)). Complement activation occurs due to the inability to inactivate C3(H2O) from tickover or the C3b produced by C3 convertases. Both C3 and factor B levels are severely depressed in complete deficiencies.
Precautions/Toxicity/Hazards
This protein is purified from human serum, therefore precautions appropriate for handling any blood-derived product must be used even though the source was shown by certified tests to be negative for HBsAg, HTLV-I/II, STS, and for antibodies to HCV, HIV-1 and HIV-II.
Hazard Code: B WGK Germany 3
MSDS available upon request.
规格
产品名称
Factor I
规格或纯度
高性能,PBS Only,1.0 mg/mL, 0.22 µm filtered
生物活性
Cleaves C3b with factor H as a cofactor
CAS编号和信息
80295-66-5
分子类型
蛋白质
产品线
生物产品
储存与运输
储存缓冲液
10 mM sodium phosphate, 145 mM NaCl, pH 7.2
浓度
1.0 mg/mL, 0.22 µm filtered
储存条件
-80℃储存,避免反复冻融
运输条件
特低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于?80 °C条件下保存。避免反复冻融。建议按单次用量分装保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 80295-66-5。
什么是「高性能」？
「高性能」是一个明确定义的分析纯度级别，适用于通用分析和试剂应用。它规定了最低含量百分比、对常见杂质（水分、重金属、灼烧残渣）的受控限值，以及批间一致性。「高性能」是几个纯度级别之一——请根据方法的灵敏度和监管要求进行选择。
什么是「PBS Only」？
「PBS Only」是一个明确定义的分析纯度级别，适用于需要特定验证属性的工作流程。它规定了最低含量百分比、对常见杂质（水分、重金属、灼烧残渣）的受控限值，以及批间一致性。「PBS Only」是几个纯度级别之一——请根据方法的灵敏度和监管要求进行选择。
本产品如何运输？
本产品采用干冰配冷袋冷冻运输。收货后请立即拆包，并按上述储存条件保存；处理干冰时请戴隔热手套并保持通风。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）