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弗林蛋白酶

Name: 弗林蛋白酶
SKU: F755284
Availability: InStock

CAS: 141760-45-4 货号: F755284

COA

有货

级别和纯度: 生物活性 ? 重组 ? ActiBioPure™ ? 高性能 ? EnzymoPure™ ? expressed in Baculovirus-Sf9 Cells,2,000 U/ml

Accession #

P09958

表达系统

Array

生物活性

2,000 U/ml

规格

库存

价格

数量

50U

F755284-50U

现货

¥3,999.90

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概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,生物活性,重组,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

Furin is a ubiquitous subtilisin-like proprotein convertase. It is the major processing enzyme of the secretory pathway and is localized in the trans-golgi network. Substrates of Furin include blood clotting factors, serum proteins and growth factor receptors such as the insulin-like growth factor receptor. The minimal cleavage site is Arg-X-X-Arg'. However, the enzyme prefers the site Arg-X-(Lys/Arg)-Arg'. An additional arginine at the P6 position appears to enhance cleavage. Furin is inhibited by EGTA, α1- Antitrypsin Portland and polyarginine compounds.

Source: Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying truncated human furin.

Assay Conditions for Unit Activity

Two fold dilutions of Furin are incubated with 25 µg MBP-FN-paramyosin-ΔSal substrate in 20 mM HEPES, 0.1% Triton X-100, 1 mM CaCl2, 0.2mM β-mercaptoenthanol (pH 7.5 @ 25°C) in a 25 µl reaction. The reaction mix is incubated at 25°C for 6 hours. Separation of reaction products are visualized by SDS-PAGE.

规格

产品名称

弗林蛋白酶

英文别名

规格或纯度

生物活性, 重组, ActiBioPure™, 高性能, EnzymoPure™, expressed in Baculovirus-Sf9 Cells,2,000 U/ml

生化机理

Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif. Mediates processing of TGFB1, an essential step in TGF-beta-1 activation. Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32). By mediating processing of accessory subunit ATP6AP1/Ac45 of the V-ATPase, regulates the acidification of dense-core secretory granules in islets of Langerhans cells (By similarity). Post-translational: The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation. Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.

生物活性

2,000 U/ml

种属

人(Human)

来源

重组表达

预测分子量

52.7 kDa

CAS编号和信息

141760-45-4

酶学委员会编号

3.4.21.75

分子类型

酶

产品线

生物产品

储存与运输

物理形态

液体

储存缓冲液

10 mM MES, 1 mM CaCl2, 50% Glycerol, pH 7 @ 25°C

浓度

expressed in Baculovirus-Sf9 Cells,2,000 U/ml

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃（12个月）；收货后建议分装，避免反复冻融。

单位定义

1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-FN-paramyosin-ΔSal substrate to 95% completion in 6 hours at 25°C in a total reaction volume of 25 µl.