技术文章

108 common laboratory cell lines

· · 发布于 2025年12月3日 · 更新于 2025年12月3日 ·

No.	Cell line	Species	Cell type	Growth pattern	Commonly used media	Commonly used serum
1	HEK293	Human	Fetal kidney epithelial-like cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
2	HepG2.2.15	Human	Hepatocellular carcinoma cells	Adherent, epithelial-like	DMEM	15% FBS
3	12TAg	Mouse	Pluripotent embryonic stem cell-like	Embryonic-type colonies	DMEM	10% FBS
4	293/CHE-Fc	Human	Kidney epithelial-like cells	Adherent, epithelial-like	DMEM	10% FBS
5	3T3-L1	Mouse	Embryonic fibroblasts / preadipocytes	Adherent, fibroblast-like	DMEM	10% FBS
6	3T6	Mouse	Embryonic fibroblasts	Adherent, fibroblast-like	DMEM	10% FBS
7	A431	Human	Epidermoid squamous cell carcinoma	Adherent, epithelial-like	DMEM	10% FBS
8	A549	Human	Lung carcinoma epithelial cells	Adherent, epithelial-like	F-12 (Kaighn’s)	10% FBS
9	A9	Mouse	Connective tissue fibroblasts	Adherent, fibroblast-like	DMEM	10% FBS
10	AR42J	Rat	Pancreatic tumor epithelial-like cells	Adherent, epithelial-like	Ham’s F-12K	20% FBS
11	AtT-20	Mouse	Pituitary tumor cells	Adherent, epithelial-like	F-10	15% HS + 2.5% FBS
12	B95-8	Marmoset	EBV-infected lymphoid-like cells	Suspension, lymphoid-like	RPMI-1640	10% FBS
13	BALB/3T3	Mouse	Embryonic fibroblasts	Adherent, fibroblast-like	DMEM	10% FBS
14	Bel-7402	Human	Hepatocellular carcinoma cells	Adherent, epithelial-like	RPMI-1640	15% FBS
15	BeWo	Human	Choriocarcinoma epithelial cells	Adherent, epithelial-like	F-12K	15% FBS
16	BHK-21	Hamster	Kidney fibroblast-like cells	Adherent, fibroblast-like	MEM + NEAA	10% FBS
17	HBL-100	Human	Mammary epithelial cells	Adherent, epithelial-like	McCoy’s 5A	10% FBS
18	BRL-3A	Rat	Liver epithelial cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
19	BT	Bovine	Turbinate bone–derived fibroblasts	Adherent, fibroblast-like	DMEM	10% FBS
20	Caco-2	Human	Colonic adenocarcinoma epithelial cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
21	Chang	Human	Liver epithelial cells	Adherent, epithelial-like	BME	10% FCS
22	CHO-K1	Hamster	Ovarian epithelial cells	Adherent, epithelial-like	F-12	10% FBS
23	CL2	Mouse	B cells (hybridoma-like)	Suspension, lymphocyte-like	RPMI-1640	10% FBS
24	CL3	Mouse	B cells (hybridoma-like)	Suspension, lymphocyte-like	RPMI-1640	10% FBS
25	Clone 9	Rat	Liver epithelial cells	Adherent, epithelial-like	F-12K	10% FBS
26	Clone M-3	Mouse	Melanoma cells	Adherent, epithelial-like	F-12	15% HS + 2.5% FBS
27	COS-1	African green monkey	Kidney fibroblast-like cells	Adherent, fibroblast-like	DMEM	10% FBS
28	COS-3	Monkey	Kidney fibroblast-like cells	Adherent, fibroblast-like	MEM	10% FBS
29	COS-7	African green monkey	Kidney fibroblast-like cells	Adherent, fibroblast-like	DMEM	10% FBS
30	CRFK	Cat	Kidney epithelial cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
31	CV-1	African green monkey	Kidney fibroblast-like cells	Adherent, fibroblast-like	MEM + NEAA	10% FBS
32	D-17	Dog	Osteosarcoma cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
33	DAN	Dog	Osteosarcoma cells	Adherent, epithelial-like	MEM/EBSS	8% FBS
34	Daudi	Human	Burkitt lymphoma B cells	Suspension, lymphoid-like	RPMI-1640	10% FBS
35	DLD-1	Human	Colorectal adenocarcinoma cells	Adherent, epithelial-like	RPMI-1640	10% FBS
36	DSDh	Dog	Osteosarcoma cells	Adherent, epithelial-like	MEM/EBSS	8% FBS
37	DSN	Dog	Osteosarcoma cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
38	Du145	Human	Prostate carcinoma cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
39	EB-3	Human	Burkitt lymphoma B cells	Suspension, lymphoid-like	RPMI-1640	10% FBS
40	ES-D3	Mouse	Pluripotent embryonic stem cells	Embryonic-type colonies	DMEM	15% FBS
41	F9	Mouse	Testicular teratocarcinoma embryonal-like cells	Embryonic to endoderm-like	DMEM	15% FBS
42	GH1	Rat	Pituitary tumor cells	Adherent, epithelial-like	F-12	15% HS + 2.5% FBS
43	GH3	Rat	Pituitary tumor cells	Adherent, epithelial-like	F-12	15% HS + 2.5% FBS
44	H9	Human	T-cell lymphoma cells	Suspension, lymphoblast-like	RPMI-1640	10% FBS
45	H9/HTLV-IIIB	Human	T-cell lymphoma (HIV/HTLV-infected)	Suspension, lymphoblast-like	RPMI-1640	10% FBS
46	HaK	Hamster	Kidney epithelial cells	Adherent, epithelial-like	BME	10% FCS
47	HCT-15	Human	Colorectal adenocarcinoma cells	Adherent, epithelial-like	RPMI-1640	10% FBS
48	HeLa	Human	Cervical carcinoma epithelial cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
49	HeLa S3	Human	Cervical carcinoma subclone	Adherent, epithelial-like	F-12	10% FBS
50	Hep-2	Human	Laryngeal carcinoma epithelial cells (HeLa-like)	Adherent, epithelial-like	MEM	10% FBS
51	Hep3B	Human	Hepatocellular carcinoma cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
52	HepG2	Human	Hepatocellular carcinoma cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
53	HK-2	Human	Renal cortical epithelial cells	Adherent, epithelial-like	Keratinocyte SFM	Serum-free (with rhEGF etc.)
54	HL-60	Human	Acute promyelocytic leukemia cells	Suspension, granulocyte-like	RPMI-1640	20% FBS
55	HP1 (HT1080 poly)	Human	Fibrosarcoma cells	Adherent, epithelial-like	DMEM	10% FBS
56	HT-1080	Human	Fibrosarcoma cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
57	HT-29	Human	Colon adenocarcinoma cells	Adherent, epithelial-like	DMEM/F-12 (1:1)	5% FBS
58	HTC	Rat	Liver epithelial cells	Adherent, epithelial-like	MEM/EBSS	10% FBS
59	HuT 78	Human	T-cell lymphoma cells	Suspension, lymphoblast-like	IMDM	20% FBS
60	HUVEC	Human	Umbilical vein endothelial cells	Adherent, epithelial-like	F-12K	10% FBS + heparin 100 μg/mL
61	HX (HT108 xeno)	Human	Fibrosarcoma (HT1080 xenograft–derived)	Adherent, epithelial-like	DMEM	10% FBS
62	I-10	Mouse	Testicular tumor cells	Adherent, epithelial-like	F-10	15% HS + 2.5% FBS
63	IEC-6	Rat	Normal small intestinal epithelial-like cells	Adherent, epithelial-like	DMEM	5% FBS + insulin 0.1 μg/mL
64	IM-9	Human	Bone marrow B cells from multiple myeloma patient	Suspension, lymphoid-like	RPMI-1640	10% FBS
65	IMR-90	Human	Lung fibroblasts	Adherent, fibroblast-like	MEM + NEAA	10% FBS
66	JAA-F11	Mouse	B-cell hybridoma	Suspension, lymphoid-like	DMEM + NEAA	10% FBS
67	JEG-3	Human	Choriocarcinoma epithelial cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
68	Jensen	Rat	Sarcoma fibroblast-like cells	Adherent, fibroblast-like	McCoy’s 5A	5% FBS
69	Jurkat	Human	Acute T-cell leukemia cells	Suspension, lymphoid-like	RPMI-1640	10% FBS
70	K-562	Human	Chronic myelogenous leukemia cells	Suspension, lymphoblast-like	RPMI-1640	10% FBS
71	KB	Human	Oral carcinoma epithelial cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
72	KG-1	Human	Erythroleukemia bone marrow cells	Suspension, myeloblast-like	IMDM	20% FBS
73	KG-1a	Human	KG-1 subline bone marrow cells	Suspension, myeloblast-like	IMDM	20% FBS
74	L2	Rat	Lung epithelial cells	Adherent, epithelial-like	F-12K	10% FBS
75	L6	Rat	Skeletal muscle myoblasts	Adherent, myoblast-like	DMEM	10% FBS
76	LLC-WRC 256	Rat	Carcinoma cells (Walker 256–derived)	Adherent, epithelial-like	199 medium	5% HS
77	LnCap	Human	Prostate carcinoma cells	Adherent, epithelial-like	RPMI-1640	10% FBS
78	LO2	Human	Normal liver epithelial cells	Adherent, epithelial-like	DMEM	15% FBS
79	M. dunni (Clone IIIBC)	Mouse	Skin fibroblasts	Adherent, fibroblast-like	McCoy’s 5A	10% FBS
80	MARC S5	Mouse	B-cell hybridoma	Suspension, lymphoid-like	MEM + NEAA or DMEM	20% heat-inactivated FBS
81	MCF-7	Human	Breast carcinoma epithelial cells	Adherent, epithelial-like	MEM + NEAA	10% FBS + insulin 10 μg/mL
82	MHCC97	Human	Hepatocellular carcinoma cells	Adherent, epithelial-like	DMEM	15% FBS
83	MOLT-4	Human	Acute T-lymphoblastic leukemia cells	Suspension, lymphoid-like	RPMI-1640	10% FBS
84	NIH3T3	Mouse	Embryonic fibroblasts	Adherent, fibroblast-like	DMEM	15% FBS
85	NK-92	Human	NK-cell lymphoma	Suspension, lymphoblast-like	α-MEM	12.5% HS + 12.5% FBS (+ IL-2)
86	NK-92MI	Human	NK-92 autocrine IL-2 variant	Suspension, lymphoblast-like	α-MEM	12.5% HS + 12.5% FBS
87	PANC-1	Human	Pancreatic carcinoma epithelial cells	Adherent, epithelial-like	DMEM	10% FBS
88	PC12	Rat	Adrenal pheochromocytoma cells	Loosely adherent, easily aggregated	F-12	15% HS + 2.5% FBS
89	PC3	Human	Prostatic adenocarcinoma (bone metastasis)	Adherent, epithelial-like	F-12	10% FBS
90	Raji	Human	Burkitt lymphoma B cells	Suspension, lymphoid-like	RPMI-1640	10% FBS
91	Rat2	Rat	Embryonic fibroblasts (Rat2 clone)	Adherent, fibroblast-like	DMEM	5% FBS + BrdU 30 μg/mL
92	RS4;11	Human	Acute lymphoblastic leukemia cells	Suspension, lymphoblast-like	RPMI-1640	10% FBS
93	Saos-2	Human	Osteosarcoma epithelial-like cells	Adherent, epithelial-like	McCoy’s 5A	15% FBS
94	SMMC7721	Human	Hepatocellular carcinoma cells	Adherent, epithelial-like	RPMI-1640	15% FBS
95	SP2/0	Mouse	Myeloma hybridoma parent line	Suspension, hybridoma-like	IMDM	7.5% FBS
96	SW480	Human	Colon carcinoma epithelial cells	Adherent, epithelial-like	L-15	10% FBS
97	SW620	Human	Colon carcinoma epithelial cells	Adherent, epithelial-like	L-15	10% FBS + 2 mM L-glutamine
98	THP-1	Human	Acute monocytic leukemia cells	Suspension, monocyte-like	RPMI-1640	10% FBS
99	U937	Human	Histiocytic lymphoma / monocytic leukemia cells	Suspension, monocyte-like	RPMI-1640	10% FBS
100	Vero	African green monkey	Kidney epithelial cells	Adherent, epithelial-like	DMEM	10% FBS
101	Vero-E6	African green monkey	Kidney epithelial cells	Adherent, epithelial-like	MEM/EBSS	10% FBS
102	WEHI-3	Mouse	Myelomonocytic leukemia cells	Suspension, macrophage-like	IMDM	10% FBS
103	WI-38	Human	Embryonic lung fibroblast / epithelial-like cells	Adherent, epithelial-like	BME	10% FBS
104	WISH	Human	Amniotic epithelial cells	Adherent, epithelial-like	BME	10% FBS
105	WS1	Human	Embryonic skin epithelial cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
106	WSS-1 (WS-1)	Human	Kidney epithelial cells	Adherent, epithelial-like	DMEM	10% FBS
107	XC	Rat	Sarcoma epithelial-like cells	Adherent, epithelial-like	MEM + NEAA	10% FBS
108	Y-1	Mouse	Adrenal tumor epithelial cells	Adherent, epithelial-like	F-10	15% HS + 2.5% FBS

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Da — 若无特别说明，分子量单位默认为道尔顿。 Mw — 重均分子量。 Mn — 数均分子量。

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阿拉丁科学.《108 common laboratory cell lines》. 阿拉丁知识库，更新于 2025年12月3日。
                            https://www.aladdin-e.com/zh_cn/faqs/108-common-laboratory-cell-lines-en.html

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