After removal from the liquid nitrogen tank, the cell cryogenic vials will occasionally burst. How to avoid it?

When performing cell cryopreservation, please strictly follow our product instructions and keep the cryogenic vials in the gas phase of the liquid nitrogen tank to avoid immersion into the liquid phase of the liquid nitrogen tank to prevent liquid nitrogen from entering the cryogenic vials. Generally, the cracking of frozen tubes is caused by liquid nitrogen entering the tube during the preservation process. After the tube is removed from the liquid nitrogen tank when the cells are resuscitated, the external temperature rises sharply, and the vaporization volume of liquid nitrogen in the tube expands rapidly, leading to the cracking of the tube, which is not a product quality problem.