Isoform controls help eliminate nonspecific staining, so how do you set the door?

We suggested using FMO combined isotype control as a negative control to go through the trap door, but FMO combined isotype control is more complicated, so many experimenters choose a simple method - using only isotype control as a negative control to go through the trap door. The isotype control is used to eliminate background staining due to non-specific binding of antibodies to help find true negative cell populations.

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