Osteosarcoma, as the most important life-threatening malignant bone tumor, is increasing in incidence. The establishment of a scientific and effective nude mouse model of human osteogenic sarcoma is one of the most important tools to overcome this disease.

Principle

The basic principle of [Nude mouse model of human osteogenic sarcoma]:

At present, human tumor xenografts in nude mice have been widely used in experimental research as a common means of establishing tumor models. By injecting human osteosarcoma cells into the bone marrow of nude mice, we can not only establish a stable nude-mouse-Holstein osteosarcoma model, but also its biological characteristics are close to those of human osteosarcoma, which is of great research and application value, and has become one of the most commonly used models of osteosarcoma.

Operation method

A nude mouse model of human osteogenic sarcoma

Principle

The basic principle of [nude mouse and human osteogenic sarcoma model] is that human tumor xenotransplantation in nude mice has been widely used in experimental research as a common tumor modeling method. By injecting human osteosarcoma cells into the bone marrow of nude mice, we can not only establish a stable nude-mouse-Holstein osteosarcoma model, but also its biological characteristics are close to those of human osteosarcoma, which is of great research and application value, and has become one of the most commonly used models for osteosarcoma.

Materials and Instruments

Equipment: Nude mice, 7-gauge syringe needle
Reagents:
① Human osteosarcoma cell line (MG63)
② Hanks solution
③ Physiological saline

Move

The basic modeling method of the nude mouse Dutch human osteogenic sarcoma model can be divided into the following steps:
A. Select nude mice as the model.
B. Select the human osteogenic sarcoma cell line (MG63), and inoculate 0.2 ml cell suspension containing ^3×106 cells into the subcutaneous of nude mice at the age of 4 weeks, and wait for the grafted tumor to enlarge to about 2 cm × 1.5 cm × 2 cm.
C. Take 1 ^cm3 of tumor tissue without necrosis, cut it into pieces, and rinse repeatedly with Hanks' solution. The tissue was washed repeatedly with Hanks' solution, centrifuged and precipitated, and then diluted with saline to 2. ^5×105/ul.
D. The above diluted solution was injected into the bone marrow cavity of the lower femur of nude mice at 6-8 weeks of age with a 7-gauge needle, and 0.2 ml was injected into each animal.
E. Twenty-four to twenty-six days after the implantation, the molybdenum-palladium X-ray film showed that the bone was destroyed, and it infiltrated into the soft tissues around the implanted tumor. 40 days later, the tumor could be pathologically fractured.

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