Check whether there are incompatible antigens and antibodies in the donor and recipient blood. This experiment is derived from the clinical experiment guide of the undergraduate 5-year program of Mudanjiang Medical College.

Operation method

ABO blood group cross-matching test

Principle

Certain blood group antigens of the red blood cells of the donor and recipient may agglutinate with the corresponding antibodies in saline medium

Materials and Instruments

Salt water
Centrifuge Microscope Test tubes

Move

I. Experimental operation: take 3-4 ml of venous blood from each recipient, separate the serum, and take the respective red blood cell area to form a 5% suspension.

Reactants (drops) Supervisor Subtube

PS + DC DS + PC Self-control

Recipient serum 1 1

Donor serum 1

5% recipient RBC 1 1

5% donor RBC 1

Second, the results of the experiment to determine: mix the above test tube immediately to 1000 γ / min centrifugation 1min removed, first look at the supernatant with or without hemolysis, and then gently flick. Observe the tubes with the naked eye, and then observe under low magnification microscope, main,

The primary and secondary test tubes were free of hemolysis and agglutination, which indicated that blood transfusion was not contraindicated.

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