Master the principle and technique of ABO blood group determination, this experiment source Mudanjiang Medical College

Operation method

ABO blood typing test

Principle

Red blood cell blood group antigen binds specifically to the corresponding antibody and an agglutination reaction occurs, which is used to determine the ABO blood group by the positive and negative typing test.

Materials and Instruments

Subject's serum Erythrocyte saline suspension

Move

I. Experimental reagents:

1, anti-A, anti-B and anti-A + B typing blood type

2, 5% A, B, O control red blood cell saline suspension

3、Serum of the examinee

4、Subjects 5% red blood cell saline suspension

Second, the experimental operation:

1, take a clean small test tube 3, respectively, labeled anti-A, anti-B and anti-A + anti-B, with a dropper, respectively, to add anti-A anti-B anti-A + B typing serum each drop in the bottom of the test tube, and then a dropper, respectively, to add the subject 5% erythrocyte saline suspension of 1 drop, mix well.

2, another take clean small test tube 3. Labeled A, B, O erythrocytes, with a dropper were added to the examinee serum l drop in the bottom of the test tube, and then were added to the dropper, respectively, A, B, O 5% control erythrocyte saline suspension of a drop, mix well.

3、Centrifuge the sample at 1000γ/min for 1 minute.

4, take out the test tube, gently shake, so that the red blood cells sunk in the bottom of the test tube floated, the first visual observation of agglutination, hemolysis phenomenon, such as the naked eye did not see the rise of the collection, should be tilted to the reaction on a clean slide with low magnification to observe whether there is agglutination phenomenon.

V. Experimental results.

Typing serum ten examinee red blood cells Subject blood type examinee serum ten reagent red blood cells

Anti-A Anti-B Anti-A + B Judgment A cell B cell O cell

+ - + A - + -

- + + + B + - - -

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