Biological Background of ACOD1

ACOD1, also known as Aconitate Decarboxylase 1 (formerly called IRG1 in mice), is a mitochondrial enzyme that catalyzes the decarboxylation of cis-aconitate to produce itaconate. Itaconate is a key immunometabolite that accumulates in activated macrophages and plays a central role in the innate immune response. By modulating succinate dehydrogenase activity and exerting direct antimicrobial effects, itaconate links cellular metabolism to immune regulation.

ACOD1 expression is strongly induced by pro-inflammatory stimuli, including LPS and cytokines such as IFN-γ. Its upregulation in macrophages during infection and inflammation makes it a compelling target for research into immunometabolism, host defense mechanisms, and inflammatory disease pathways.

Product Specifications

The ACOD1 Mouse mAb (SKU: Ab351137) is a monoclonal antibody produced in mouse. The antibody is formulated in phosphate-buffered saline (PBS) without the addition of preservatives or carrier proteins, making it compatible with a wide range of downstream applications.

• Host Species: Mouse
• Antibody Type: Monoclonal Antibody (mAb)
• Formulation: PBS only
• Target: ACOD1 / Aconitate Decarboxylase 1
• SKU: Ab351137

The PBS-only formulation is ideal for researchers who require additive-free preparations for conjugation, functional assays, or specialized experimental setups. Storage and handling conditions should be confirmed upon receipt of the product datasheet.

Recommended Research Applications

The ACOD1 Mouse mAb is suitable for research applications requiring specific detection of the ACOD1 protein. Typical applications for monoclonal antibodies with this target and formulation profile include:

• Western Blotting (WB): Detection of ACOD1 protein expression in cell lysates and tissue extracts from macrophage or inflammatory models.
• Immunofluorescence (IF): Visualization of ACOD1 subcellular localization in fixed cells or tissue sections.
• Immunoprecipitation (IP): Enrichment of ACOD1 protein complexes for interaction studies.
• ELISA: Quantitative detection of ACOD1 in research-grade assay formats.

Researchers are advised to validate the antibody in their specific experimental system. Confirmed application data and optimal working dilutions should be referenced from the official product datasheet provided by Aladdin Scientific.

Handling, Storage, and Quality Considerations

Proper handling and storage are essential to maintain the activity and specificity of the ACOD1 Mouse mAb. As the antibody is supplied in a PBS-only formulation without stabilizing additives, the following general guidelines apply:

• Store the antibody at -20°C for long-term preservation. Avoid repeated freeze-thaw cycles.
• Upon first use, aliquot the antibody into single-use volumes to minimize degradation.
• Keep the antibody on ice during experimental procedures.
• Do not expose to prolonged light or elevated temperatures.

Please refer to the product-specific datasheet for confirmed storage conditions, expiry information, and lot-specific quality control data. For technical support, contact Aladdin Scientific's customer service team.