Target Biology: What Is ANGPTL1?

ANGPTL1 (Angiopoietin-Like Protein 1) is a secreted glycoprotein belonging to the angiopoietin-like protein family. It shares structural homology with the angiopoietins, featuring an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, yet it does not bind the Tie receptors that classical angiopoietins engage.

ANGPTL1 is expressed in a variety of tissues including liver, heart, and vascular endothelium. Research has implicated ANGPTL1 in the regulation of angiogenesis, with evidence suggesting it can inhibit endothelial cell migration, tube formation, and tumor neovascularization. Its role in lipid metabolism and potential involvement in cancer progression and suppression have made it a subject of active investigation.

A high-quality monoclonal antibody against ANGPTL1 enables researchers to detect, localize, and study this protein with precision across multiple experimental platforms.

Product Description & Formulation

ANGPTL1 Mouse mAb (SKU: Ab351151) is a monoclonal antibody generated in mouse host against the ANGPTL1 target antigen. The antibody is supplied exclusively in PBS (phosphate-buffered saline) formulation without additional carrier proteins or preservatives unless otherwise specified at time of purchase.

The PBS-only format makes this antibody well-suited for applications requiring minimal background from excipients, and it is compatible with direct conjugation workflows where addition of BSA or azide would be undesirable. Researchers should note that the absence of stabilizing proteins may require particular attention to storage conditions to maintain antibody integrity over time.

Reference supplier catalog information: absea catalog reference KC-41844. Please verify current lot-specific details including concentration, isotype, and validated applications on the Aladdin Scientific product page for Ab351151.

Recommended Applications & Protocol Guidance

Monoclonal antibodies against ANGPTL1 are commonly employed in the following research applications. Researchers should validate the antibody for each specific application in their own experimental system:

• Western Blot (WB): Detect ANGPTL1 protein expression in cell lysates or tissue homogenates under denaturing conditions. Optimize antibody dilution empirically, typically starting in the range of 1:500–1:2000.
• Immunohistochemistry (IHC): Localize ANGPTL1 expression in formalin-fixed, paraffin-embedded (FFPE) or frozen tissue sections. Antigen retrieval may be required.
• Immunofluorescence (IF): Visualize subcellular distribution of ANGPTL1 in fixed cell preparations.
• Enzyme-Linked Immunosorbent Assay (ELISA): Quantify ANGPTL1 levels in capture or sandwich ELISA formats.
• Immunoprecipitation (IP): Pull down ANGPTL1 and associated protein complexes for downstream analysis.

Note: Confirmed validated applications and recommended dilutions for Ab351151 should be verified from the current product datasheet available on the Aladdin Scientific website.

Storage, Handling & Stability

Proper storage and handling of PBS-formulated monoclonal antibodies are essential to preserve activity and ensure reproducible experimental results.

• Short-term storage (up to 1 month): Store at 4°C. Avoid repeated freeze-thaw cycles.
• Long-term storage: Aliquot into single-use volumes and store at -20°C or -80°C. Adding a carrier protein such as BSA (0.1%) is recommended if long-term storage stability is a concern and downstream applications permit.
• Avoid: Repeated freeze-thaw cycles (limit to fewer than 5), exposure to direct light, and contamination with proteases.
• Reconstitution: If supplied lyophilized, reconstitute with the volume of sterile water or PBS as specified on the Certificate of Analysis.

Always centrifuge briefly before opening to recover any material from the cap. Check the Certificate of Analysis (CoA) supplied with each lot for lot-specific concentration, purity data, and expiration date.

Frequently Cited Research Contexts for ANGPTL1

ANGPTL1 has been studied across several biomedical research domains where a reliable detection antibody is essential:

Tumor Biology & Angiogenesis: Multiple studies have investigated ANGPTL1 as a potential suppressor of tumor angiogenesis. Its reported capacity to inhibit endothelial cell migration and capillary tube formation positions it as a candidate anti-angiogenic factor, relevant to cancer research and therapeutic target discovery.

Lipid & Metabolic Research: Members of the ANGPTL family, including ANGPTL1, have been examined for roles in lipid metabolism, lipoprotein lipase regulation, and metabolic syndrome-related pathways.

Cardiovascular Biology: Given its expression in heart and vascular tissues, ANGPTL1 is relevant to studies of cardiac remodeling and endothelial function.

Researchers working in any of these contexts will benefit from a high-specificity monoclonal antibody such as Ab351151 to accurately detect and quantify ANGPTL1 in their experimental models. Always include appropriate isotype controls and validate results with orthogonal methods where possible.