Target Biology: Apolipoprotein E (APOE)

Apolipoprotein E (APOE) is a multifunctional glycoprotein that plays a central role in lipid transport and metabolism. It is synthesized primarily in the liver and brain, where it mediates the uptake of lipoprotein particles via cell-surface receptors including the low-density lipoprotein receptor (LDLR) family. In the central nervous system, APOE is the predominant apolipoprotein produced by astrocytes and is critically involved in neuronal lipid homeostasis, synaptic remodeling, and injury response.

APOE exists in three major human isoforms — APOE2, APOE3, and APOE4 — encoded by distinct alleles of the APOE gene. The APOE4 isoform is the strongest known genetic risk factor for late-onset Alzheimer's disease, making APOE a high-priority target in neurodegenerative disease research. Reliable antibody-based detection of APOE protein is therefore essential for studies ranging from basic lipid biology to clinical biomarker research.

Product Specifications

APOE Mouse mAb (Ab351153) is a murine monoclonal antibody raised against human apolipoprotein E. The antibody is supplied in phosphate-buffered saline (PBS) without carrier proteins or preservatives unless otherwise stated, making it compatible with a wide range of downstream conjugation and labeling procedures.

• Catalog SKU: Ab351153
• Host Species: Mouse
• Antibody Class: Monoclonal
• Formulation: PBS
• Supplier Cross-Reference: absea #KC-1219

Note: Specific details such as isotype, clone name, reactivity species, exact concentration, and validated applications are not fully described in the current source material. Researchers should consult the official Aladdin Scientific product datasheet or contact technical support for complete specifications prior to experimental use.

Recommended Applications

Monoclonal antibodies against APOE are broadly employed in standard immunological assays. While researchers should verify validated applications in the official Ab351153 datasheet, anti-APOE monoclonal antibodies of this class are commonly used in the following techniques:

• Western Blot (WB): Detection of APOE in cell lysates, tissue homogenates, and plasma/serum fractions under denaturing conditions.
• Enzyme-Linked Immunosorbent Assay (ELISA): Quantitative measurement of APOE levels in biological fluids and conditioned media.
• Immunohistochemistry (IHC): Localization of APOE expression in formalin-fixed, paraffin-embedded (FFPE) or frozen tissue sections.
• Immunofluorescence (IF): Visualization of APOE distribution in cultured cells or tissue sections using fluorescent secondary antibodies.
• Immunoprecipitation (IP): Isolation of APOE protein complexes for co-precipitation and interaction studies.

Optimal antibody dilutions and conditions should be determined empirically for each application and sample type.

Storage, Handling & Quality Considerations

Proper storage and handling of monoclonal antibodies is essential to maintain activity and ensure reproducible experimental results. General best-practice guidelines for PBS-formulated antibodies include:

• Short-term storage: Store at 4 °C for up to one month when the antibody will be used frequently.
• Long-term storage: Aliquot and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can degrade antibody performance.
• Avoid: Repeated freeze-thaw cycles, exposure to light for extended periods, and contamination from non-sterile pipettes or tubes.
• Carrier protein-free formulation: Because this product is supplied in PBS only, researchers who require stabilization for long-term storage may consider adding a carrier protein (e.g., BSA) and/or a preservative (e.g., sodium azide) at their discretion, consistent with downstream application requirements.

Always consult the official product datasheet for lot-specific storage instructions and expiration information before use.