Product Overview: ASB3 Mouse mAb

The ASB3 Mouse mAb (SKU: Ab351159) is a mouse-derived monoclonal antibody raised against ASB3, also known as Ankyrin Repeat and SOCS Box Protein 3. ASB3 is a member of the ASB family of proteins characterized by ankyrin repeat domains and a C-terminal SOCS box motif, which plays roles in ubiquitin-mediated proteasomal degradation pathways. This antibody is supplied in a PBS-only formulation, making it suitable for downstream conjugation or direct use in aqueous-based assay systems.

This product is referenced under absea catalog number KC-36556 and is offered by Aladdin Scientific to support research into protein degradation, cell signaling, and related biological pathways.

Formulation, Storage, and Handling

Formulation: ASB3 Mouse mAb (Ab351159) is supplied in PBS (Phosphate-Buffered Saline) only, without additives such as glycerol, BSA, or sodium azide unless otherwise stated on the specific lot Certificate of Analysis.

Storage: As a general guideline for monoclonal antibodies in PBS, it is recommended to store the antibody at 4°C for short-term use (up to one month) and at -20°C or -80°C for long-term storage. Repeated freeze-thaw cycles should be avoided; aliquoting is strongly recommended upon first use.

Handling: Keep the antibody on ice during experiments. Centrifuge briefly before opening the vial to collect any residual liquid from the cap. Do not expose to repeated freeze-thaw cycles, as this may compromise antibody integrity and performance.

Note: Specific storage temperature, expiry, and concentration details should be confirmed on the product's Certificate of Analysis (CoA). Contact Aladdin Scientific technical support for lot-specific documentation.

Intended Applications and Validation

ASB3 Mouse mAb is intended for use in standard research applications where monoclonal antibodies against ASB3 are required. Typical applications for antibodies of this class may include, but are not limited to:

• Western Blot (WB): Detection of ASB3 protein in cell or tissue lysates.
• Immunoprecipitation (IP): Pull-down of ASB3 protein complexes from cellular extracts.
• Immunofluorescence (IF): Localization of ASB3 within fixed cells or tissue sections.
• ELISA: Quantitative detection of ASB3 in suitable sample matrices.

Users should verify the suitability of this antibody for each specific application in their own experimental system. Validated applications and recommended dilutions for this specific lot should be confirmed with the product's datasheet or by contacting Aladdin Scientific technical support.

Note: Specific validated applications are not detailed in the current source material. This section reflects general antibody application guidance. Review flags have been raised accordingly.

Ordering, Availability, and Technical Support

ASB3 Mouse mAb is available from Aladdin Scientific under SKU Ab351159. To place an order, visit the Aladdin Scientific website, search by SKU or product name, and proceed through the standard purchasing workflow. Bulk quantities, custom packaging, or institutional pricing inquiries should be directed to the Aladdin Scientific sales team.

For technical questions regarding antibody performance, experimental optimization, or application-specific guidance, contact Aladdin Scientific technical support with your lot number and Certificate of Analysis at hand. Providing details of your experimental system, sample type, and observed results will help expedite troubleshooting assistance.

For order tracking, returns, or billing inquiries, please refer to Aladdin Scientific's customer service portal or contact the relevant department directly.

Troubleshooting Common Issues

Below are general troubleshooting guidelines applicable to ASB3 Mouse mAb and similar monoclonal antibodies in PBS formulation:

• No signal detected: Verify that the antibody has been stored correctly and has not undergone repeated freeze-thaw cycles. Check that the appropriate secondary antibody (anti-mouse) is being used. Optimize primary antibody concentration.
• High background: Increase blocking stringency (e.g., use 5% non-fat dry milk or BSA). Increase wash steps and duration. Titrate the primary antibody to a lower concentration.
• Non-specific bands (Western Blot): Optimize antibody dilution. Confirm sample preparation and denaturation conditions. Consider using a positive control lysate known to express ASB3.
• Weak signal: Increase primary antibody incubation time or concentration. Ensure secondary antibody and detection reagents are appropriate and active.

If problems persist, contact Aladdin Scientific technical support with your experimental details for further guidance.