Blood collection site: WHO recommends that peripheral blood should be taken from the inside of the tip of the middle finger or ring finger. In some places in China, earlobe blood is still taken, but its blood circulation is poor. It is affected by the temperature. The conditions of blood collection are not as constant as fingers. Therefore, attention should be paid to local massage and improvement of blood circulation when picking earlobes. Infants under half a year old earlobes and fingers are too small, usually to the thumb or root of the foot to take.

Operation method

Blood specimen collection experiments

Materials and Instruments

Blood collection needles Three-bladed needles Syringes Tourniquets

Move

1. Capillary blood collection method

Blood collection site: WHO recommends taking peripheral blood to the inner side of the tip of the middle finger or ring finger. In some places in China, earlobe blood is still collected, but its blood circulation is poor. It is affected by the temperature. The conditions of blood collection are not as constant as fingers. Therefore, attention should be paid to local massage and improvement of blood circulation when picking earlobes. Infants under half a year old earlobes and fingers are too small, usually to the thumb or root of the foot to take.

I. Experimental equipment: blood collection needles, three needles with blades, to ensure that one person a needle, in order to prevent cross-infection.

Second, the experimental steps: 75% alcohol cotton balls to disinfect the local skin, to be volatile alcohol, blood collection needle into the 2-3mm, to slightly squeeze the blood can flow out as appropriate, according to the need for blood collection in turn. Blood collection is completed, with disinfection of dry cotton ball pressure on the wound for a few moments can be.

Third, the experimental precautions:

The selected site for blood collection should not have frostbite, redness, edema, inflammation, etc. For multiple tests, the order of specimens taken should be hematocrit, red blood cell count, hemoglobin self-assessment and white blood cell count. A separate needle is required for coagulation time measurement.

2. Venous blood collection method:

Blood collection site: elbow vein. The back of the hand, young children can use the external jugular vein.

I. Experimental equipment: 5ml syringe, tourniquet.

Experimental steps: the patient takes the lying position or sitting position, arm straight and flat on the whole pillow. Expose the puncture site. After finding a good vein for blood collection, sterilize it with 75% alcohol, hold the syringe in the right hand, make the needle bevel and cylinder scale upward, and rapidly pierce the skin at a position of 30 degrees. Puncture into the center of the venous cavity, see the return of blood immediately after fixing the syringe, so as not to slip out of the blood vessels, at this time with the left hand slowly pumping the syringe needle bolt to the required amount of blood after lifting the tourniquet, relax the fist, with a sterile dry cotton ball pressure puncture hole, pull out the needle, remove the needle, the blood along the wall of the test tube slowly injected into the test tube, let it coagulate, to be after the blood clots contraction, can be separated from both the yellowish transparent serum. If whole blood or plasma is required, the blood is injected into the pre-prepared anticoagulation tube and gently traced. Prevent coagulation, that is, anticoagulated blood. Third, the experimental notes: blood can only be drawn outward. It can not be pushed into the vein, so as not to inject air into the formation of air embolism, resulting in serious consequences.

Caveat

The site selected for blood collection should not have frostbite, purplish redness, edema, inflammation, etc. For multiple tests, the order of specimens taken is hematocrit, red blood cell count, hemoglobin self-assessment, and white blood cell count. A separate needle is required for coagulation time measurement.

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