A large amount of suggestive data can be obtained by testing blood, so blood routine and blood biochemistry in mice are widely used in phenotyping and have become important tests. Currently, there are 3 main methods used for blood testing experiments: blood smear, blood routine and blood biochemistry.

Operation method

blood smear

Materials and Instruments

Equipment:
Slides, fresh blood samples.

Move

The basic procedure for blood smears can be divided into the following steps:

A Using two clean slides with smooth edges, drop a freshly separated blood sample directly onto nearly 1/3 of one of the slides, usually 5 to 10 μl, and then touch the edge of the other slide to the blood to make a line of blood and then push it away from the other side at an angle of 30° to 45° in a uniform and stable manner, and then push it out uniformly to form a successful blood smear, which can be used for staining and counting of blood cells (Fig. 8-1-5).

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