Recipe

1.Dissolve 50mg of Coomassie Blue G250 in 50ml of methanol

2.Add 100ml of 85% H3PO4 to the solution from step 1

3.Add the solution from step 2 into 500ml of H2O and mix

4.Filter to remove and precipitates

5.Add an additional 350ml of H2O

6.Store at 4℃.

Caution: Always add acid slowly into water and do not add water into acid.