Overview of C22orf31 Mouse mAb

C22orf31 Mouse mAb (Ab350891) is a monoclonal antibody raised in mouse against the C22orf31 protein, an open reading frame gene product located on human chromosome 22. The antibody is formulated in phosphate-buffered saline (PBS) without additional preservatives or carrier proteins unless otherwise specified. As with all research-grade monoclonal antibodies, it is intended exclusively for in vitro research use and is not suitable for diagnostic or therapeutic applications in humans.

Researchers should consult the relevant literature for the known or proposed biological functions of C22orf31, as the functional characterization of this protein may be evolving. This guide provides practical handling, storage, and application instructions to help ensure reproducible results.

Storage and Handling

Proper storage and handling are critical to maintaining antibody activity and ensuring experimental reproducibility. Follow these guidelines for C22orf31 Mouse mAb:

• Short-term storage (up to 1 month): Store at 4°C. Avoid repeated freeze-thaw cycles, which can denature the antibody and reduce its binding affinity.
• Long-term storage: Aliquot the antibody upon receipt and store at −20°C or −80°C. Single-use aliquots minimize freeze-thaw-related degradation.
• Formulation: This product is supplied in PBS only. If your application requires a different buffer, dialysis or buffer exchange may be performed, though care should be taken not to dilute the antibody excessively.
• Avoid: Prolonged exposure to light, temperatures above 25°C, and repeated freeze-thaw cycles. Do not use if the solution appears turbid or contains visible particulates.

Always centrifuge briefly before opening the vial to collect any material from the cap or walls.

Recommended Protocol for Common Applications

The C22orf31 Mouse mAb may be applicable in standard immunoassay techniques. Researchers should perform empirical optimization for their specific system, sample type, and detection method. The following general guidelines apply:

Western Blot (WB)

1. Prepare cell or tissue lysates using a standard lysis buffer containing protease inhibitors.
2. Resolve proteins by SDS-PAGE and transfer to a PVDF or nitrocellulose membrane.
3. Block the membrane with 5% non-fat dry milk or BSA in TBST for 1 hour at room temperature.
4. Incubate with C22orf31 Mouse mAb at the recommended dilution (to be determined empirically; a typical starting dilution is 1:500–1:2000) overnight at 4°C.
5. Wash three times with TBST (10 minutes each).
6. Incubate with an appropriate HRP-conjugated anti-mouse secondary antibody for 1 hour at room temperature.
7. Wash three times with TBST and develop using a chemiluminescent substrate.

Immunofluorescence (IF) / Immunohistochemistry (IHC)

1. Fix and permeabilize cells or tissue sections according to standard procedures.
2. Block with 5–10% normal serum from the secondary antibody host species.
3. Incubate with C22orf31 Mouse mAb at the appropriate dilution (optimization required).
4. Apply a fluorophore-conjugated or enzyme-conjugated anti-mouse secondary antibody.
5. Counterstain and mount as appropriate.

Note: Optimal working dilutions and conditions must be determined by the end user for each specific application and sample type. Always include appropriate positive and negative controls.

Troubleshooting

If you experience unexpected results when using C22orf31 Mouse mAb, consider the following troubleshooting steps:

Always verify that your sample expresses C22orf31 at the expected level before concluding that the antibody is non-functional.

Quality, Safety and Regulatory Notes

C22orf31 Mouse mAb (Ab350891) is manufactured and quality-controlled as a research-use-only reagent. The following points should be noted by all users:

• Research Use Only (RUO): This product is not approved for diagnostic, therapeutic, or clinical use.
• Lot-to-lot variability: Minor batch variations may occur. Users are encouraged to validate each new lot against a previous lot if consistency is critical.
• Biosafety: Handle the antibody using standard laboratory precautions for biological reagents. Consult your institution's biosafety guidelines.
• Disposal: Dispose of unused product and contaminated materials in accordance with local environmental and institutional regulations.
• Expiry and QC: Refer to the product datasheet for the specific lot expiration date and quality control data. Contact Aladdin Scientific technical support for additional documentation.