常见问题(FAQs)
Can cell lysate lysed cells be followed by immunoprecipitation?
Can cell lysate lysed cells be followed by immunoprecipitation?
Can cell lysate lysed cells be followed by immunoprecipitation?
No. Cell lysate for immunoprecipitation is a lysate that lyses cells under non-denaturing conditions. Conventional RIPA lyses contain SDS, which can denature proteins and affect protein interactions. Lysates that do not contain denaturing agents are recommended for immunoprecipitation.
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