Most of Candida albicans need oxygen 25-30 ℃ to grow well, can also be in 37 ℃ above the growth. Colonies are cream-colored, pie-shaped and smooth. In the corn - Tween 80 agar plate, 25 ℃ 72 hours under the microscope morphology: pseudohyphae septum accompanied by clusters of rounded conidia, the tip of the thick-walled thick membrane spores, bud tube test positive. With the bacteria in the morphology of similar strains of Candida astrocytica, the difference between the two is that the latter does not assimilate sucrose, not fermented galactose.

Operation method

Candida albicans identification test

Principle

Most of Candida albicans need oxygen 25-30 ℃ to grow well, can also be in 37 ℃ above the growth. Colonies are cream-colored, pie-shaped and smooth. In the corn - Tween 80 agar plate, 25 ℃ 72 hours under the microscope morphology: pseudohyphae septum accompanied by clusters of rounded conidia, the tip of the thick-walled thick membrane spores, bud tube test positive. With the bacteria in the morphology of similar strains of Candida astrocytica, the difference between the two is that the latter does not assimilate sucrose, not fermented galactose.

Move

Culture: Inoculate the specimen on Sabouraud's medium at 36℃ for 18-24 hours, the colony is cream-colored, yeast-like, with pseudohyphae and budding spores under the microscope.

Identification test:

(1) bud tube test: take a small amount of Candida albicans to be examined inoculated in 0.5 ml of human or rabbit serum, mixed, placed in a 37 ℃ water bath for 2-3 h, take out an inoculation ring of bacterial fluid, smear, microscopic examination. Candida albicans is the main producer of germ tubes, other candida do not produce germ tubes (a few star candida can also produce germ tubes). Candida albicans is the one that produces bud tubes,

(2) TZC test: Inoculate Candida albicans seedlings in Sabouraud's medium containing 0.005% triphenyltetrazolium chloride (TZC), incubate at 22-25℃ for 24-48 hours, Candida albicans will not change color or only light red, other Candida or yeasts will be red or deep red.

(3) Sugar fermentation and sugar assimilation test: According to these two tests, several common species of Candida can be identified.

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