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Operation method

Chemical sterilization experiment

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1. Lysol: It exerts bactericidal effect through the mechanism of damaging cell membrane, inactivating enzymes and denaturing proteins. The formula is 500g of cresol, 173g of vegetable oil, 27g of sodium hydroxide, add water to 1000ml. the concentration of disinfection is 2%, used for disinfecting the ground, the surface of the utensils, the skin and so on. It can effectively kill bacterial propagules, fungi, Mycobacterium tuberculosis and inactivate most viruses, but it cannot kill bacterial spores, and the inactivation effect on hepatitis B virus is not certain.

2、Xinjermei: It can play the role of sterilization through the mechanism of damaging cell membrane, inactivating the activity of oxidative enzymes and precipitating proteins, etc. The concentration of disinfection is 0.05%. The concentration of disinfection is 0.05%～0.10%, which is used for surgical hand washing, immersion of surgical instruments, disinfection of skin and mucous membranes, and disinfection of wound rinsing. It has strong bacteriostatic effect but weak bactericidal effect, it has inhibition or killing effect on many kinds of bacteria, it has no killing effect on Mycobacterium tuberculosis and bacterial spores; it has inactivation effect on influenza virus, cowpox virus, herpes virus, etc., but it has poor inactivation effect on most of the intestinal viruses, and it can't inactivate Hepatitis B virus.

3、Peroxyacetic acid: through oxidation, destruction of bacterial enzyme system and acidification and other mechanisms to play a bactericidal effect. Conventional formula for glacial acetic acid: hydrogen peroxide: sulfuric acid is equal to 1.0: 0.5: 0.05. Disinfection concentration is 0.2% ~ 0.3%. It is used for the disinfection of articles contaminated by excreta and thermometers, tongue depressors, dialyzers, tableware, plastics, glass equipment, and room air. It has high efficiency and rapid killing effect on bacterial propagules, fungi, tuberculosis bacillus, bacterial spores, various viruses and so on.

4, alcohol: through protein denaturation, coagulation, interference with metabolism and other mechanisms to play a bactericidal effect. Sterilization concentration of 70% ~ 75%. It is used for the disinfection of hand, skin, smooth object surface and skin disinfection deiodization. Bacterial propagules, fungi, most viruses have a strong killing effect, but can not kill bacterial spores.

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