Principles and Techniques of Coagulation Time Measurement from Mudanjiang Medical College

Operation method

Coagulation time test experiment

Principle

When blood leaves the body and comes into contact with a negatively charged surface (glassware), E.D. factors are activated, followed by a series of coagulation factors that are successively activated to convert fibrinogen to fibrin, a process known as the coagulation time.

Materials and Instruments

Test tubes Syringes Water baths

Move

1、Take 2 clean test tubes with inner diameter of 8mm.

2, with a sterilized syringe intravenous blood collection 2mL injected into the prepared test tubes, each tube 1ml, static 37 ℃ water bath.

3, placed in the water bath for 5 minutes, every half a minute to tilt the test tube, until the blood coagulation (inverted test tube blood does not flow), while observing the second tube, according to the average time of the two tubes of blood coagulation report.

4. The time from the time the blood is injected into the syringe until the blood does not flow is the clotting time.

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