What Is Decarbonylation Colchicine?

Decarbonylation colchicine is a synthetic analog of the natural alkaloid colchicine, differing by the removal of the carbonyl (C=O) group from the tropolone C-ring. Colchicine itself is a well-characterized inhibitor of tubulin polymerization, binding at the colchicine site on β-tubulin and preventing microtubule assembly. The decarbonylated derivative is used in research contexts primarily to investigate how this carbonyl moiety contributes to binding affinity, selectivity, and biological potency at the colchicine binding site.

As a research reagent (Aladdin SKU D768317), Decarbonylation colchicine provides scientists with a tool to conduct structure–activity relationship (SAR) studies, helping to define the pharmacophoric requirements of colchicine-site ligands without the full pharmacological profile of the parent compound.

Structural Comparison with Colchicine

Colchicine is composed of three rings: a trimethoxyphenyl A-ring, a seven-membered B-ring bearing an acetamido group, and a tropolone C-ring containing a carbonyl and a methoxy substituent. Decarbonylation colchicine retains the A- and B-ring framework but lacks the carbonyl functionality on the C-ring.

This structural simplification is scientifically significant: the carbonyl group of colchicine's tropolone ring participates in key interactions with residues in the colchicine binding pocket of tubulin. Removal of this group is therefore expected to modulate (typically reduce) binding affinity, making the compound a valuable negative-control or SAR probe in comparative tubulin-binding assays.

Note: Specific binding affinity data (K_d values) for Decarbonylation colchicine versus colchicine should be verified against primary literature; no specific figures are provided in the current product documentation.

Research Applications and Handling

Research Applications: Decarbonylation colchicine is primarily employed in:

• Structure–activity relationship (SAR) studies at the colchicine binding site of tubulin
• Comparative binding assays (e.g., competitive displacement assays using fluorescent colchicine analogs)
• Mechanistic investigation of tubulin–ligand interactions
• Serving as a reference compound in antimitotic drug discovery programs

Solubility and Formulation: Like colchicine, this analog is expected to be soluble in common organic solvents such as DMSO and ethanol. Aqueous solubility may be limited. Researchers should prepare stock solutions in DMSO and dilute into aqueous buffers or cell culture media immediately before use. Final DMSO concentration in biological assays should be kept ≤0.1% to minimize solvent effects. (Verify solubility data against the product Certificate of Analysis.)

Safety: Colchicine and its analogs are toxic compounds. Although Decarbonylation colchicine may have altered pharmacological activity relative to colchicine, it should be handled with appropriate caution: use gloves, work in a well-ventilated area or fume hood, and consult the Safety Data Sheet (SDS) before use.

Storage, Stability, and Quality

Recommended Storage: Store Decarbonylation colchicine at −20 °C in a tightly sealed container, protected from light and moisture. As with most alkaloid derivatives, repeated freeze–thaw cycles should be avoided to preserve compound integrity.

Stability: The compound is expected to be stable under the recommended storage conditions for an extended period; however, researchers should verify the expiry date and lot-specific stability data provided on the Certificate of Analysis (CoA) supplied with each order.

Purity and Quality: Aladdin Scientific reagents are quality-controlled prior to release. The purity specification and analytical data (e.g., HPLC, NMR, MS) for SKU D768317 are available on the product CoA. Researchers requiring high-purity material for quantitative binding studies should confirm purity ≥95% on the CoA before use.

Note: The source description references a related product at McLean (麦克林) catalog number D885897. Researchers should confirm they are ordering the correct product from Aladdin (D768317) and cross-reference specifications as needed.

Ordering and Support

How to Order: Decarbonylation colchicine (SKU D768317) is available from Aladdin Scientific. To place an order, visit the Aladdin Scientific website, search by SKU D768317 or product name, and add to cart. For bulk quantities or custom requirements, contact the Aladdin sales team directly.

Technical Support: For technical questions about this product—including solubility, formulation recommendations, or application-specific guidance—please contact Aladdin Scientific's technical support team via the website or by email. Our specialists can assist with protocol development and reagent selection.

Documentation: The Safety Data Sheet (SDS) and Certificate of Analysis (CoA) for each lot are available for download from the product page or upon request from customer service.