How can l detect the Dil-labeled liposomes?

Control liposomes labeled with the fluorochrome DiI, or DiI-labeled liposomes, can be used to investigate whether the liposomes have reached the targeted macrophage population via the chosen administration protocol. The fluorescent label will show the distribution pattern of the liposomes within tissues and their uptake by macrophages. DiI-labeled liposomes can be detected either by fluorescent microscopy of flow cytometry. The DiI-molecule’s excitation and emission is 549 and 565 nm respectively (see figure).